Protein Info for RS_RS01625 in Ralstonia solanacearum GMI1000

Annotation: C4-dicarboxylate transporter 1

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 428 transmembrane" amino acids 7 to 26 (20 residues), see Phobius details amino acids 41 to 65 (25 residues), see Phobius details amino acids 77 to 99 (23 residues), see Phobius details amino acids 150 to 168 (19 residues), see Phobius details amino acids 188 to 209 (22 residues), see Phobius details amino acids 223 to 244 (22 residues), see Phobius details amino acids 308 to 337 (30 residues), see Phobius details amino acids 348 to 374 (27 residues), see Phobius details PF00375: SDF" amino acids 9 to 401 (393 residues), 380.3 bits, see alignment E=5.6e-118

Best Hits

Swiss-Prot: 100% identical to DCTA1_RALSO: C4-dicarboxylate transport protein 1 (dctA1) from Ralstonia solanacearum (strain GMI1000)

KEGG orthology group: K11103, aerobic C4-dicarboxylate transport protein (inferred from 100% identity to rso:RSc0330)

MetaCyc: 62% identical to C4 dicarboxylate/orotate:H+ symporter (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-121; TRANS-RXN-121A; TRANS-RXN-121C; TRANS-RXN-122A; TRANS-RXN0-451; TRANS-RXN0-517; TRANS-RXN0-553

Predicted SEED Role

"Aerobic C4-dicarboxylate transporter for fumarate, L-malate, D-malate, succunate, aspartate"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8Y2K5 at UniProt or InterPro

Protein Sequence (428 amino acids)

>RS_RS01625 C4-dicarboxylate transporter 1 (Ralstonia solanacearum GMI1000)
MKKPFYKILYVQVLAAIVIGVLLGHFEPKLAVNMKPLGDGFIQLIKMVIGPIIFCTVVSG
IAGMRDMKKVGRVGGKALLYFEVVSTFALVIGLVAGHIFNPGSGFNVDVNSIDAKAVAQY
AAKAQSSSTVDFLLNIIPSTVVDAFAKGDILQILLIALLFGGALSAMGERAQMVTDFIDQ
IAHVFFRIVHVITRVAPIGAFGAMAFTIGKYGVVSLVPLLKLIGTFYLTAIIFVVVVLGI
IARLTGFSIFRFVAYIKEELLIVLGTSSSESALPHLMEKMEKLGCSKSVVGLVVPTGYSF
NLDGTNIYMTMAVIFISQALNIELTLTQQLTILAVAMLTSKGASGITGAGFITLAATLAV
VPTIPVAGMVLILGIDRFMSECRALTNITGNGVACVVISAWERELDRTKLARVMSGDRGE
TVSATPAA