Protein Info for RS_RS00995 in Ralstonia solanacearum GMI1000

Annotation: MBL fold metallo-hydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 452 transmembrane" amino acids 238 to 257 (20 residues), see Phobius details PF00753: Lactamase_B" amino acids 17 to 194 (178 residues), 52.6 bits, see alignment E=1.5e-17 PF16661: Lactamase_B_6" amino acids 19 to 172 (154 residues), 30.1 bits, see alignment E=7.5e-11 PF12706: Lactamase_B_2" amino acids 24 to 232 (209 residues), 41.8 bits, see alignment E=2.3e-14 PF10996: Beta-Casp" amino acids 247 to 366 (120 residues), 109.4 bits, see alignment E=4e-35 PF07521: RMMBL" amino acids 380 to 443 (64 residues), 67.5 bits, see alignment E=2.1e-22

Best Hits

KEGG orthology group: K07576, metallo-beta-lactamase family protein (inferred from 100% identity to rso:RSc0201)

Predicted SEED Role

"Metallo-beta-lactamase family protein, RNA-specific" in subsystem Bacterial RNA-metabolizing Zn-dependent hydrolases

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8Y2Y0 at UniProt or InterPro

Protein Sequence (452 amino acids)

>RS_RS00995 MBL fold metallo-hydrolase (Ralstonia solanacearum GMI1000)
MQLQFLGATETVTGSKYVLETGARRVMVDCGLFQGYKSLRLRNWDTLAVNPHHIDAVVLT
HAHIDHSGYLPLLVRNGFRGPVYCTKGTAELCNILLPDSARLAVEDAQYANAQGFSRHHP
ALPLYDEQDAAKALRRLHPVGYGERFSVVDGVEAEFHRAGHIIGAATVTLLAEGRRTVFS
GDLGRQVDPVMRPPEPVAEADTLLVESTYGDRLHPPGDPLDELERIVHRTIEQGGTLLIP
AFAVGRTQALLFCLYTLIRTHRIPHVPIYLDSPMAERATEVFARHVEELHIDAADCQAAC
ALAVPVQSPQQSMHLGNDRAPKIILAASGMATGGRVLHHLKSFGGGKRNAILMSGFQAAG
TRGAALLAGQRQLRVHGREIAIRATVEQIANLSAHADANELMAWLRGFSRAPEQTFIVHG
EPAASDALRQRIERELQWPVRMPEYRQSYALS