Protein Info for RS_RS00865 in Ralstonia solanacearum GMI1000

Annotation: tRNA 2-thiocytidine(32) synthetase TtcA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 312 PF01171: ATP_bind_3" amino acids 34 to 196 (163 residues), 56.4 bits, see alignment E=1.8e-19

Best Hits

Swiss-Prot: 100% identical to TTCA_RALSO: tRNA-cytidine(32) 2-sulfurtransferase (ttcA) from Ralstonia solanacearum (strain GMI1000)

KEGG orthology group: K14058, tRNA 2-thiocytidine biosynthesis protein TtcA (inferred from 100% identity to rso:RSc0175)

MetaCyc: 70% identical to tRNA cytosine32 2-sulfurtransferase TtcA (Escherichia coli K-12 substr. MG1655)
2.8.1.M2 [EC: 2.8.1.M2]

Predicted SEED Role

"tRNA(Cytosine32)-2-thiocytidine synthetase"

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.8.1.M2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8Y306 at UniProt or InterPro

Protein Sequence (312 amino acids)

>RS_RS00865 tRNA 2-thiocytidine(32) synthetase TtcA (Ralstonia solanacearum GMI1000)
MTFSNNFHRLETRLQSQAGRAIGDFKMIEDGDTVLVCLSGGKDSYTMLSVLMALQKRAPI
DFKLIAMNLDQKQPGFPEDVLPGYLKKVGVEYVIVEADTYSIVKEKVPEGKTTCSLCSRL
RRGVIYRTAKELGANKIALGHHRDDIVNTFFLNMFFGGKMKAMPPKLATDNGDHIVIRPL
AYCAEKEIAAYARAMEFPIIPCNLCGSQENLQRKKVKEMLLEWERQAPGRIDNIFSALQN
VVPSHLADTDLFDFNGLTTGLAKIGEEALFGQTAYDQAPLVFAGSHDDRIEFVRFERNPA
GKAPEGAEQPAA