Protein Info for RS_RS00535 in Ralstonia solanacearum GMI1000

Annotation: thiazole synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 275 PF05690: ThiG" amino acids 15 to 261 (247 residues), 357.1 bits, see alignment E=2.3e-111

Best Hits

Swiss-Prot: 100% identical to THIG_RALSO: Thiazole synthase (thiG) from Ralstonia solanacearum (strain GMI1000)

KEGG orthology group: K03149, thiamine biosynthesis ThiG (inferred from 100% identity to rso:RSc0109)

Predicted SEED Role

"Thiazole biosynthesis protein ThiG" in subsystem Thiamin biosynthesis

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8Y372 at UniProt or InterPro

Protein Sequence (275 amino acids)

>RS_RS00535 thiazole synthase (Ralstonia solanacearum GMI1000)
MTTTNLSALADPLRLYDEVFGSRLLLGTARYPSPQSLEDAVRACGPAMLTVALRRQSAGT
PEGGAGFWKMLRALGVPVLPNTAGCYSADEAYTLAQMSREVFETDWIKLEVIGDDYTLQP
DTLALPAAAERLIRDGFKVLPYCTEDLVLCRRLLDVGCRALMPWAAPIGTGRGPTNPYGL
RLLRERLPDVPLIVDAGLGVPSHATQVMEWGYDGVLLNTAVAQAGDPVAMARAFAMATQA
GRLARLSGPMPERDVAQASTPVVGLPFWHADKNSA