Protein Info for RS_RS00530 in Ralstonia solanacearum GMI1000
Annotation: thiamine phosphate synthase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
KEGG orthology group: K00788, thiamine-phosphate pyrophosphorylase [EC: 2.5.1.3] (inferred from 100% identity to rso:RSc0108)Predicted SEED Role
"Thiamin-phosphate pyrophosphorylase (EC 2.5.1.3)" in subsystem Thiamin biosynthesis (EC 2.5.1.3)
MetaCyc Pathways
- superpathway of thiamine diphosphate biosynthesis II (9/11 steps found)
- thiamine diphosphate biosynthesis I (E. coli) (2/2 steps found)
- thiamine diphosphate biosynthesis II (Bacillus) (2/2 steps found)
- thiamine diphosphate salvage II (4/5 steps found)
- thiamine diphosphate salvage V (2/3 steps found)
- superpathway of thiamine diphosphate biosynthesis I (7/10 steps found)
- thiamine diphosphate biosynthesis III (Staphylococcus) (1/3 steps found)
- thiamine diphosphate biosynthesis IV (eukaryotes) (1/3 steps found)
- superpathway of thiamine diphosphate biosynthesis III (eukaryotes) (3/7 steps found)
- thiamine diphosphate salvage IV (yeast) (3/7 steps found)
- thiamine diphosphate formation from pyrithiamine and oxythiamine (yeast) (3/8 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 2.5.1.3
Use Curated BLAST to search for 2.5.1.3
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See Q8Y373 at UniProt or InterPro
Protein Sequence (383 amino acids)
>RS_RS00530 thiamine phosphate synthase (Ralstonia solanacearum GMI1000) MSASALRFASAWPDAAALADRLIDRHADAFGRAPHAWSVTDRADDAATAAVLLTTDAAQA ERARAAGAAVVLSEARNGERIDTVHDRLGTYRFAAPATGAVFGERFVAMFGAALALAFEP RDALCVARAWIAEAAADALAWPARFDALPRVLEPALPCAASPDLAFAPCPPRLGVYAVVP DAEWVERLVALKVPTVQLRVKSDDARAVAGQVRRAAAAARGSQTRLFINDHWRVALDVHA QTPDSGLYGIHLGQEDIDDADLAAIRASGLRLGISTHGYAEMLRVAALNPSYLALGAVFA TPTKTMPTLPQGLGRLFAHAAAMRSRVPAPPLVAIGGIDLAAMPRVLESGVGGVAVVRAI TQAEDVPAAVQALQATFAAHARA