Protein Info for RS_RS00415 in Ralstonia solanacearum GMI1000
Annotation: aspartate aminotransferase family protein
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 47% identical to FUSA3_GIBZE: Aminotransferase FGSG_17085 (FGRAMPH1_01T09365) from Gibberella zeae (strain PH-1 / ATCC MYA-4620 / FGSC 9075 / NRRL 31084)
KEGG orthology group: None (inferred from 100% identity to rso:RSc0082)MetaCyc: 34% identical to lysine-8-amino-7-oxononanoate aminotransferase (Bacillus subtilis)
RXN-14930 [EC: 2.6.1.105]
Predicted SEED Role
"Adenosylmethionine-8-amino-7-oxononanoate aminotransferase (EC 2.6.1.62)" in subsystem Biotin biosynthesis (EC 2.6.1.62)
MetaCyc Pathways
- biotin biosynthesis I (13/15 steps found)
- biotin biosynthesis from 8-amino-7-oxononanoate I (4/4 steps found)
- biotin biosynthesis from 8-amino-7-oxononanoate II (4/4 steps found)
- biotin biosynthesis II (5/6 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 2.6.1.62
Use Curated BLAST to search for 2.6.1.105 or 2.6.1.62
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See Q8Y398 at UniProt or InterPro
Protein Sequence (450 amino acids)
>RS_RS00415 aspartate aminotransferase family protein (Ralstonia solanacearum GMI1000) MTPSTTSVFHRDPRKTLPTAVGGESMMLIDAEGKRYLDASGGAAVSCLGHGHPRVVEAIC KQVRALAYAHTSFFTTAVAEELAARLAAAAPGDLGHVYFVSGGSEAIEAALKLARQYFVE IGQPQRQYFIARRQSYHGNTLGALAIGGNAWRREPFLPLLVPAHHVSPCFAYRERRDGET DAQYVQRLADELDAKIVELGADRVAAFIAETVVGATAGAVPPVADYFRKIRAVCDRHGVL LILDEVMSGMGRTGHLFACEEDGVVPDLIAIAKGLGGGYQPIGAMIASDRIYDAITRGSG FFQHGHTYIGHATACAAALEVQKVIAEENLLANVLARGEQLRAALRERFAAHPHVGDVRG RGLFVGVELVADRGTKAPLPPARKTHARVRAEAMQRGLMVYPMGGTIDGQRGDHILLAPP FIATAQDIDAIVARLGDAVEAATANALEAA