Protein Info for RS_RS00290 in Ralstonia solanacearum GMI1000

Annotation: Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase GatCAB subunit C

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 100% identical to GATC_RALSO: Glutamyl-tRNA(Gln) amidotransferase subunit C (gatC) from Ralstonia solanacearum (strain GMI1000)

KEGG orthology group: K02435, aspartyl-tRNA(Asn)/glutamyl-tRNA (Gln) amidotransferase subunit C [EC: 6.3.5.6 6.3.5.7] (inferred from 100% identity to rso:RSc0058)

MetaCyc: 32% identical to glutamyl-tRNA(Gln) amidotransferase, C subunit (Synechococcus elongatus PCC 7942 = FACHB-805)
Glutaminyl-tRNA synthase (glutamine-hydrolyzing). [EC: 6.3.5.7]; Asparaginyl-tRNA synthase (glutamine-hydrolyzing). [EC: 6.3.5.7, 6.3.5.6]

Predicted SEED Role

"Aspartyl-tRNA(Asn) amidotransferase subunit C (EC 6.3.5.6) @ Glutamyl-tRNA(Gln) amidotransferase subunit C (EC 6.3.5.7)" (EC 6.3.5.6, EC 6.3.5.7)

MetaCyc Pathways

L-citrulline biosynthesis (8/8 steps found)
L-glutamate and L-glutamine biosynthesis (7/7 steps found)
superpathway of L-citrulline metabolism (10/12 steps found)
L-asparagine biosynthesis III (tRNA-dependent) (4/4 steps found)
ammonia assimilation cycle III (3/3 steps found)
L-glutamate biosynthesis I (2/2 steps found)
L-glutamine degradation I (1/1 steps found)
glutaminyl-tRNA^gln biosynthesis via transamidation (3/4 steps found)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.3.5.6, 6.3.5.7

Use Curated BLAST to search for 6.3.5.6 or 6.3.5.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8Y3C2 at UniProt or InterPro

Protein Sequence (99 amino acids)

>RS_RS00290 Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase GatCAB subunit C (Ralstonia solanacearum GMI1000)
MALELSDVKRIAHLARIEVSDGEAAQTLTQLNQFFTLVEQMQAVDTTGIEPMAHPIEQVQ
APAQRLREDAVTEPDRRADYQQCAPSTEAGLYLVPKVIE