Protein Info for RR42_RS36205 in Cupriavidus basilensis FW507-4G11

Annotation: amidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 502 PF01425: Amidase" amino acids 38 to 476 (439 residues), 296.5 bits, see alignment E=2.1e-92

Best Hits

KEGG orthology group: K01426, amidase [EC: 3.5.1.4] (inferred from 73% identity to reh:H16_B1874)

Predicted SEED Role

"Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases"

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.5.1.4

Use Curated BLAST to search for 3.5.1.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0C4YG35 at UniProt or InterPro

Protein Sequence (502 amino acids)

>RR42_RS36205 amidase (Cupriavidus basilensis FW507-4G11)
MTRQDIASTDTRPPKARFLPVWEAAALLAKGDTSAQDLVNWCEAAYAASHREINALILAD
FSAARAAAEASDQRRRRGEPLGLLDGIPYSIKESFDVAGWPTTCGNPAQRHNIAISDSAV
VERLRAAGAVLLGKTNVPIGLRDWQSYNAIYGTTRNPRDPGRTPGGSSGGSAAAVCAGMS
FFDVGSDIGSSLRNPAHYCGVFSHKSSHGLVPLRGHGIGHGYGEQEINVAGPLARSAHDL
ELLLRALAGPDGIEAQAYRLTLPPCDKRTLADFRVAVLPSHPLCEADGEVSDEIEKLGRW
LEGQGAQVRWNARPDFDASALWHTYVLMLRATTSIHMDDAGFDAALARAAGAAPDDHSYA
TLQFTGAGIRHRQWLQLQQARATFAQAWQRFFGEVDVLLCPAAATTAFVLDEAGEPWQRM
LQVNGRAQPLTSQLFWAGHSGLCGLPSTVAPIGPGRSGLPVGVQIVAGKFEDLTTLRFAQ
LLEAAGFAFRPPPASAQSQSQS