Protein Info for RR42_RS32970 in Cupriavidus basilensis FW507-4G11

Annotation: benzoylformate decarboxylase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF02776: TPP_enzyme_N
  • amino acids 17 to 117 (101 residues), 83 bits, E=2.1e-27
• PF00205: TPP_enzyme_M
  • amino acids 205 to 329 (125 residues), 50.6 bits, E=2.5e-17
• PF02775: TPP_enzyme_C
  • amino acids 392 to 533 (142 residues), 109.9 bits, E=1.6e-35

Best Hits

KEGG orthology group: K01576, benzoylformate decarboxylase [EC: 4.1.1.7] (inferred from 78% identity to bcm:Bcenmc03_2607)

Predicted SEED Role

"Benzoylformate decarboxylase (EC 4.1.1.7)" in subsystem Benzoate degradation (EC 4.1.1.7)

MetaCyc Pathways

• mandelate degradation to acetyl-CoA (15/18 steps found)
• superpathway of aromatic compound degradation via 3-oxoadipate (25/35 steps found)
• D-phenylglycine degradation (1/3 steps found)
• 4-hydroxymandelate degradation (3/6 steps found)
• mandelate degradation I (2/5 steps found)
• indole-3-acetate degradation II (2/7 steps found)
• superpathway of aromatic compound degradation via 2-hydroxypentadienoate (21/42 steps found)

KEGG Metabolic Maps

• Benzoate degradation via hydroxylation
• Toluene and xylene degradation

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.1.1.7

Sequence Analysis Tools

See A0A0C4YNQ2 at UniProt or InterPro

Protein Sequence (536 amino acids)

>RR42_RS32970 benzoylformate decarboxylase (Cupriavidus basilensis FW507-4G11)
MAGAARNVTSVTSATSTVRDAVIDLLRQLGITSVFANPGSTELPMFRDFPADFRYVLGLQ
EAVVVGMADGFAQASRNASLVNLHSAAGVGNAMGNIFTAFKNRTPMIVTAGQQARSILPF
DPFLSSTQATELAKPYVKWSIEPARAADVPLAIARAYYVAMQEPRGPVLVSIPVDDWDQP
AERVAARAVSTEVRPDPVAIGRVGAALDACERPAFVIGGAVDRAGAWDDVVRLAETHNAR
VYVAPMSGRCGFPEDHRLFAGFLPAMREKIVGLLGGHDLILVLGAPAFTYHVEGAGPHVP
EGATLYQLIDDPAIAAWTPVGASVVGNIRLGVLDLLARPAPRARPLPAPRQPRSHAEPTR
LMSTAYVLQTLAEVRDAADIVVEEAPSARPVMQGYLPFTQSGTFYTMDSGGLGYGMPAAV
GVALARPGSRVIALIGDGSSMYSIQAIWSAVQLKLPITFVILNNARYAALQDFAPEFGFA
PSDPVQGTDLPGIDFVALAQGMGCEGTRVRDAARLRDALADGLRSQVPTLVEVEVA