Protein Info for RR42_RS32675 in Cupriavidus basilensis FW507-4G11

Annotation: phenol 2-monooxygenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 504 transmembrane" amino acids 189 to 210 (22 residues), see Phobius details amino acids 264 to 283 (20 residues), see Phobius details PF02332: Phenol_Hydrox" amino acids 22 to 250 (229 residues), 245 bits, see alignment E=8.2e-77 PF04945: YHS" amino acids 408 to 450 (43 residues), 60.8 bits, see alignment 1e-20

Best Hits

Swiss-Prot: 67% identical to DMPN_PSEUF: Phenol hydroxylase P3 protein (dmpN) from Pseudomonas sp. (strain CF600)

KEGG orthology group: None (inferred from 94% identity to reh:H16_B0542)

MetaCyc: 69% identical to toluene ortho-monooxygenase hydroxylase TomA3 subunit (Burkholderia cepacia)

Predicted SEED Role

"Phenol hydroxylase, P3 oxygenase component DmpN (EC 1.14.13.7)" (EC 1.14.13.7)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.14.13.7

Use Curated BLAST to search for 1.14.13.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0C4YEC2 at UniProt or InterPro

Protein Sequence (504 amino acids)

>RR42_RS32675 phenol 2-monooxygenase (Cupriavidus basilensis FW507-4G11)
MDARKKLNLREKYATMTRDLGWETTYEPMDKVFPYDKYEGIKIHDWDKWEDPFRMTMDAY
WKYQSEKERKLYAIIDSFVQNNGHLNVSDPRYLNALRLFLTGVTPLEYAAHRGYAHLGRH
FRGAGARVAAQMQSIDELRHAQTQMHTLSVYNKYFNGFGEWQHMHDRVWYLSVPKSYFED
AMSAGPFEFITAISFSFEYVLTNLLFMPFMSGAAYNGDMATVTFGFSAQSDESRHMTLGL
EVVKFLCQQDPDNIPILQKWLDKWFWRGFRLLTLVGMMMDYMLPKRVMSWAQAWEMYFEQ
AGGALFKDLERYGLRMPKYHEVATKTKDRITHEAWGTFYNYAAAAGFHTWVPKQDEMAWL
TEKYPDTFARYYKPRLDYWQERQQQGERFYNATLPMLCQTCQIPMVFSEPDDPTQTCYRE
SSYHGMRFHFCSDGCKDIFDGEPQKYAQAWLPVHQIYQGNCGGGSLEDVLKWYRINPGAD
NLDFEGSQDQKNWNAWKGVPGTAA