Protein Info for RR42_RS32650 in Cupriavidus basilensis FW507-4G11
Annotation: betaine-aldehyde dehydrogenase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 76% identical to DMPC_PSEUF: 2-hydroxymuconic semialdehyde dehydrogenase (dmpC) from Pseudomonas sp. (strain CF600)
KEGG orthology group: K10217, aminomuconate-semialdehyde dehydrogenase [EC: 1.2.1.32] (inferred from 91% identity to reh:H16_B0547)MetaCyc: 63% identical to 2-aminomuconate semialdehyde dehydrogenase (Comamonas testosteroni CNB-1)
Aminomuconate-semialdehyde dehydrogenase. [EC: 1.2.1.32]; 1.2.1.32 [EC: 1.2.1.32]
Predicted SEED Role
"Putative 5-carboxymethyl-2-hydroxymuconate semialdehyde dehydrogenase oxidoreductase protein (EC 1.2.1.60)" in subsystem Aromatic amino acid degradation (EC 1.2.1.60)
MetaCyc Pathways
- L-tryptophan degradation IX (11/12 steps found)
- L-tryptophan degradation XII (Geobacillus) (11/12 steps found)
- 2-amino-3-carboxymuconate semialdehyde degradation to 2-hydroxypentadienoate (4/4 steps found)
- 2-nitrobenzoate degradation I (6/7 steps found)
- 2-aminophenol degradation (3/4 steps found)
- 2-amino-3-carboxymuconate semialdehyde degradation to glutaryl-CoA (3/5 steps found)
- 4-hydroxyphenylacetate degradation (5/8 steps found)
- L-tryptophan degradation III (eukaryotic) (10/15 steps found)
- 4-chloronitrobenzene degradation (4/9 steps found)
- 4-nitrotoluene degradation II (4/9 steps found)
- L-tryptophan degradation XI (mammalian, via kynurenine) (11/23 steps found)
KEGG Metabolic Maps
- Benzoate degradation via hydroxylation
- Toluene and xylene degradation
- Tryptophan metabolism
- Tyrosine metabolism
Isozymes
Compare fitness of predicted isozymes for: 1.2.1.32, 1.2.1.60
Use Curated BLAST to search for 1.2.1.32 or 1.2.1.60
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A0C4YEB8 at UniProt or InterPro
Protein Sequence (484 amino acids)
>RR42_RS32650 betaine-aldehyde dehydrogenase (Cupriavidus basilensis FW507-4G11) MKEFKNFINGEWVATNRRFDDRNPVDNSLIARVHEAGQAEVDAAVAAAKQALRGPWGKMT VARRVEILHAVADGINRRFDDFLQAEIADTGKPHSLASHVDIPRGAANFKVFADLIKNVP TESFAMATPDGGEAINYAVRSPRGVIGVVCPWNLPLLLMTWKVGPALACGNTVVVKPSEE TPATATLLGEVMNEAGVPPGVYNVVHGFGPDSAGAFLTAHPDVNGITFTGETRTGEAIMK AAANGVRPVSFELGGKNAGIVFADADFDKAVAGITRSAFENSGQVCLGTERVYVQRPIFA RFVAALKARAEALRIGRPNDEGVNMGPLVSLEHRDKVLSYYRKAADAGATVVTGGGVPEM PGALAEGAWVQPTIWTGLPEDAAVIREEIFGPCCHIAPFDTEEEAIRLANDTPYGLAATV WTSDLGTAHRMGSALEVGICWINAWFLRDLRTAFGGAKQSGIGREGGVHSLEFYTELRNV CVKL