Protein Info for RR42_RS30660 in Cupriavidus basilensis FW507-4G11

Annotation: 5-methyltetrahydropteroyltriglutamate-- homocysteine methyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF08267: Meth_synt_1
  • amino acids 4 to 310 (307 residues), 410.5 bits, E=6.1e-127
  • amino acids 539 to 682 (144 residues), 28.4 bits, E=1.2e-10
• TIGR01371: 5-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase
  • amino acids 8 to 754 (747 residues), 1094.6 bits, E=0
• PF01717: Meth_synt_2
  • amino acids 427 to 749 (323 residues), 517.3 bits, E=1.7e-159

Best Hits

Swiss-Prot: 78% identical to METE_CUPNH: 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase (metE) from Cupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337)

KEGG orthology group: K00549, 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase [EC: 2.1.1.14] (inferred from 79% identity to reu:Reut_B4245)

Predicted SEED Role

"5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase (EC 2.1.1.14)" in subsystem Methionine Biosynthesis (EC 2.1.1.14)

MetaCyc Pathways

• aspartate superpathway (23/25 steps found)
• superpathway of L-methionine biosynthesis (by sulfhydrylation) (12/12 steps found)
• superpathway of L-lysine, L-threonine and L-methionine biosynthesis I (16/18 steps found)
• superpathway of L-lysine, L-threonine and L-methionine biosynthesis II (13/15 steps found)
• L-methionine biosynthesis III (4/4 steps found)
• S-adenosyl-L-methionine salvage II (3/3 steps found)
• L-methionine biosynthesis II (5/6 steps found)
• superpathway of S-adenosyl-L-methionine biosynthesis (7/9 steps found)
• superpathway of L-methionine biosynthesis (transsulfuration) (7/9 steps found)
• superpathway of L-homoserine and L-methionine biosynthesis (6/8 steps found)
• S-adenosyl-L-methionine salvage I (3/4 steps found)
• L-methionine biosynthesis I (3/5 steps found)
• seleno-amino acid biosynthesis (plants) (3/5 steps found)
• superpathway of seleno-compound metabolism (6/19 steps found)

KEGG Metabolic Maps

• Biosynthesis of plant hormones
• Methionine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.1.1.14

Sequence Analysis Tools

See A0A0C4YKS6 at UniProt or InterPro

Protein Sequence (795 amino acids)

>RR42_RS30660 5-methyltetrahydropteroyltriglutamate-- homocysteine methyltransferase (Cupriavidus basilensis FW507-4G11)
MARTHIAGFPRIGAQRELKFAQEAFWRGETDEASLRQVATGLRQRHWQSQAQAGLDSVAV
GDFAYYDQVLGLSALLGALPARFGFDPKQLSLAQYFELARGNKAQPAMEMTKWFDTNYHY
LVPELGPDTRFDGGVDWFFDEVEEALALGLKAKPVLIGPVTYLWLSKTHVDGFDRLSLLP
SLVAAYGRILARLRDRGIEWVQIDEPALCLDLEPAWLDAYDGAYAELAKSGVKLLLATYF
DNAADHAARAAALPVHGFHIDLVRAPQQLDAWRKVLPAGAVLSAGVIDGRNIWRTDLRRA
LSVLAPLHAELGERLWVAPSCSLLHVPVSLEAERKLDAQIKTWLAFATEKLAEVSVLARA
LNEGEAAVAPELAAADAALASRRSSSRVVNQQVQARVAAVTAAMAQRKSPFQTRIERQRT
ALQLPALPTTTIGSFPQTTAIRQTRAAYRRGEIGALEYLQRIRGEIETAVRKQEALGLDV
LVHGEAERNDMVEYFGEQLCGYTFTENGWVQSYGSRCVKPPVIYGDVYRPEPMTVETTRY
AQSLTERPMKGMLTGPVTMLQWSFVRDDQPRATTTLQLALAIRDEVSDLEQAGIRIIQID
EPALREGLPLRKGDWAAYLDWAVHAFKISAASVEDQTQIHTHMCYAEFNDILPAIAALDA
DVITIETSRSAMELLEGFGEFDYPNEIGPGVYDIHSPRVPSVEAITHLLERACEVIPPQR
LWVNPDCGLKTRAWPETEAALANMVAAARGLREQIGKDGATAWQRATRRTGLGGHAAHHG
AAHAAGCVDCASQAA