Protein Info for RR42_RS27650 in Cupriavidus basilensis FW507-4G11

Annotation: phosphate-binding protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 327 signal peptide" amino acids 1 to 14 (14 residues), see Phobius details PF12849: PBP_like_2" amino acids 14 to 296 (283 residues), 134.9 bits, see alignment E=4.8e-43 TIGR00975: phosphate ABC transporter, phosphate-binding protein PstS" amino acids 16 to 322 (307 residues), 378.9 bits, see alignment E=8.6e-118 PF01547: SBP_bac_1" amino acids 16 to 300 (285 residues), 52 bits, see alignment E=1.1e-17

Best Hits

Swiss-Prot: 58% identical to PSTS_XYLFT: Phosphate-binding protein PstS (pstS) from Xylella fastidiosa (strain Temecula1 / ATCC 700964)

KEGG orthology group: K02040, phosphate transport system substrate-binding protein (inferred from 68% identity to rme:Rmet_5829)

MetaCyc: 47% identical to phosphate ABC transporter periplasmic binding protein (Escherichia coli K-12 substr. MG1655)
ABC-27-RXN [EC: 7.3.2.1]; 7.3.2.1 [EC: 7.3.2.1]

Predicted SEED Role

"Phosphate ABC transporter, periplasmic phosphate-binding protein PstS (TC 3.A.1.7.1)" in subsystem High affinity phosphate transporter and control of PHO regulon or Phosphate metabolism (TC 3.A.1.7.1)

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 7.3.2.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0C4YR12 at UniProt or InterPro

Protein Sequence (327 amino acids)

>RR42_RS27650 phosphate-binding protein (Cupriavidus basilensis FW507-4G11)
MSLFASCAPCAHAGDITGAGSTFVYPLLVKWAATYYTKTGQEVNYQPTGSGNGIRQIKAA
NVTFGASDMPLKPDELRAAGLVQFPLVVGGVVPIVNLDGVAAGQIRFTGQLLADIYLGKI
ANWADPAIGAINPGIHFPNQKILVVHRSDSSGTTFNWADYLSKVSDEWKGSVGSGTTVKW
PAGVGANGNEGVATYIKNVKGAIGYVELTYALQRKLPYTAVRNRDGAFIEPARESFSAAV
ASADWSQHPDFYQVITDAPGMQAWPITGAVFVIMPKSPKDPAGARAALAFFRWALNDGQA
DAAAEHYVALPPALVKQIEAYWGENLK