Protein Info for RR42_RS26485 in Cupriavidus basilensis FW507-4G11

Annotation: 2,5-dihydroxypyridine 5,6-dioxygenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

No protein families (PFam or TIGRFam), signal peptides, or transmembrane helices were found in this protein.

Best Hits

Swiss-Prot: 56% identical to NICX_PSEPK: 2,5-dihydroxypyridine 5,6-dioxygenase (nicX) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)

KEGG orthology group: K01269, aminopeptidase [EC: 3.4.11.-] (inferred from 85% identity to reh:H16_B0813)

MetaCyc: 78% identical to 2,5-dihydroxypyridine 5,6-dioxygenase (Alcaligenes faecalis)
2,5-dihydroxypyridine 5,6-dioxygenase. [EC: 1.13.11.9]

Predicted SEED Role

"Leucyl aminopeptidase (aminopeptidase T)"

MetaCyc Pathways

picolinate degradation (7/7 steps found)
nicotinate degradation I (6/6 steps found)
nicotine degradation III (VPP pathway) (6/10 steps found)
nicotine degradation II (pyrrolidine pathway) (6/11 steps found)
superpathway of nicotinate degradation (7/18 steps found)

KEGG Metabolic Maps

Nicotinate and nicotinamide metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.4.11.-

Use Curated BLAST to search for 1.13.11.9 or 3.4.11.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0C4YQ78 at UniProt or InterPro

Protein Sequence (348 amino acids)

>RR42_RS26485 2,5-dihydroxypyridine 5,6-dioxygenase (Cupriavidus basilensis FW507-4G11)
MPVSDHALIQAWKQVLTLSRLAPGQTVTILTSAATHPQTLSCALVATQAMGAIVNRLDLP
PVNADKALSRDPLAYLGTTALTGNRAAIAALKESDLVLDLMTLLFSPEQHDILKTGTKIL
LAVEPPEVLVRMVPSEADRERVSAANARLSRAKEMTVTSDAGTDLRCPLGEFPAISEYGF
VDEPGRWDHWPSGFVLTWPNEGGASGRIVLDRGDILLPQKGYVADRVALTVERGFATRIE
GGLDAELLNDYMASFNDPQAYAISHIGWGLQPRARWSTLGLYDREASIGMDARAYEGNFL
FSLGPNNEAGGRRTTACHIDIPLRNCTVSLDGEEVVRQGKVLDGWSSK