Protein Info for RR42_RS26425 in Cupriavidus basilensis FW507-4G11

Annotation: biphenyl 2,3-dioxygenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00355: Rieske
  • amino acids 55 to 139 (85 residues), 72 bits, E=3e-24
• PF00848: Ring_hydroxyl_A
  • amino acids 206 to 427 (222 residues), 65.7 bits, E=5.7e-22

Best Hits

Swiss-Prot: 44% identical to ANDAC_BURCE: Anthranilate 1,2-dioxygenase large subunit (andAc) from Burkholderia cepacia

KEGG orthology group: K05708, large terminal subunit of phenylpropionate dioxygenase [EC: 1.14.12.19] (inferred from 90% identity to reh:H16_B0801)

MetaCyc: 78% identical to 6-hydroxypicolinate 3-monooxygenase subunit 3 (Alcaligenes faecalis)
1.14.13.-

Predicted SEED Role

"Ortho-halobenzoate 1,2-dioxygenase alpha-ISP protein OhbB" in subsystem Benzoate degradation

MetaCyc Pathways

• picolinate degradation (7/7 steps found)
• 3-phenylpropanoate and 3-(3-hydroxyphenyl)propanoate degradation (6/8 steps found)
• 3-phenylpropanoate and 3-(3-hydroxyphenyl)propanoate degradation to 2-hydroxypentadienoate (3/5 steps found)
• cinnamate and 3-hydroxycinnamate degradation to 2-hydroxypentadienoate (3/5 steps found)

KEGG Metabolic Maps

• Phenylalanine metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.14.12.19

Use Curated BLAST to search for 1.14.12.19

Sequence Analysis Tools

See A0A0C4YL78 at UniProt or InterPro

Protein Sequence (429 amino acids)

>RR42_RS26425 biphenyl 2,3-dioxygenase (Cupriavidus basilensis FW507-4G11)
MYQTHAVINRTPRAKDAELAACQWPEDALHFIPDWVYTSNAVYDLELEKIFRGRSWNFVA
LEAEIPNPGDFKRSYVGPTPVVVARAEDGSVNVFENRCAHRGAEFCRHGQGNAKEFVCPY
HQWSYDLKGNLQGVPFKRGVNRAGGMPKDFRNEDHGLVKLNVATRNGVIFASYANDMEPL
DVYMTPEILKDFDVVFNGKPLKILGYYKNELPCNWKMYHENLKDPYHATLLHSFLVVFGL
LVAGNDSAMIADPVHGRHGTMASAKKEDKYAAVSDENKKEMRSYHEGMRLQDDRFLEYIK
EFDSPWSVTMQTIWPNLIVQREMNTLGVRQIVPNGPDSMLMQWTMFGYEDDTEEMTRHRL
RQGNLMGPAGFLGLEDNEAMKFVQEGVRRSSTERNVLKLDPGHVGTANSLISESAIRAMY
QYYRSVMGF