Protein Info for RR42_RS25910 in Cupriavidus basilensis FW507-4G11
Annotation: acyl-CoA dehydrogenase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 39% identical to BBSG_THAAR: (R)-benzylsuccinyl-CoA dehydrogenase (bbsG) from Thauera aromatica
KEGG orthology group: K00249, acyl-CoA dehydrogenase [EC: 1.3.99.3] (inferred from 88% identity to reh:H16_B0913)MetaCyc: 39% identical to benzylsuccinyl-CoA dehydrogenase subunit (Thauera aromatica)
RXN-865 [EC: 1.3.8.3]
Predicted SEED Role
"Acyl-CoA dehydrogenase, short-chain specific (EC 1.3.99.2)" in subsystem Isoleucine degradation (EC 1.3.99.2)
MetaCyc Pathways
- pyruvate fermentation to butanol II (engineered) (4/6 steps found)
- pyruvate fermentation to hexanol (engineered) (7/11 steps found)
- 1-butanol autotrophic biosynthesis (engineered) (18/27 steps found)
- toluene degradation to benzoyl-CoA (anaerobic) (1/6 steps found)
- toluene degradation VI (anaerobic) (5/18 steps found)
- anaerobic aromatic compound degradation (Thauera aromatica) (7/27 steps found)
KEGG Metabolic Maps
- Butanoate metabolism
- Fatty acid metabolism
- Propanoate metabolism
- Valine, leucine and isoleucine degradation
- beta-Alanine metabolism
Isozymes
Compare fitness of predicted isozymes for: 1.3.99.2, 1.3.99.3
Use Curated BLAST to search for 1.3.8.3 or 1.3.99.2 or 1.3.99.3
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A0C4YJ47 at UniProt or InterPro
Protein Sequence (388 amino acids)
>RR42_RS25910 acyl-CoA dehydrogenase (Cupriavidus basilensis FW507-4G11) MNFVLEDEHQMLKDLVARFVREQLIPLEAGVLAREAEGGQLTLLPQEQDKLDGLSRELGL WGLDAPAEMGGADLPVVAMVGVNEELGKTVTPYDLPPDSPNLRMLLKTADAAQRARYLEP YARGETISAIAISEPGAGGDPAMMTTRAERDGDDWVLNGRKIWISRAARADWTIVMAVTD KARGARGGISAFLVDRGTPGFKVERRIPMIGGASTYEVVLEDCRVAHTQLLGVQGQGFAP MQARLSSRRVQMAAWCIGRAQRALDMLCEYAPQRRTFGAALADRQAIQWWVADAATRIHA CRLMTYEAAARIDAGDEARTQVSMIKVFATEMAWDVIDHAMQAFGAMGMTKEMPLQQMAN ETRLMRIYEGPSEVHRWVIARDLLGLKR