Protein Info for RR42_RS22745 in Cupriavidus basilensis FW507-4G11

Annotation: phosphoribosylglycinamide formyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01142: phosphoribosylglycinamide formyltransferase 2
  • amino acids 1 to 381 (381 residues), 559.6 bits, E=1.6e-172
• PF02786: CPSase_L_D2
  • amino acids 107 to 191 (85 residues), 20.2 bits, E=7.5e-08
• PF02222: ATP-grasp
  • amino acids 109 to 286 (178 residues), 165.4 bits, E=1.9e-52
• PF07478: Dala_Dala_lig_C
  • amino acids 116 to 289 (174 residues), 32.3 bits, E=1.4e-11
• PF21244: PurT_C
  • amino acids 312 to 380 (69 residues), 86.1 bits, E=1.8e-28

Best Hits

Swiss-Prot: 91% identical to PURT_CUPMC: Formate-dependent phosphoribosylglycinamide formyltransferase (purT) from Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)

KEGG orthology group: K08289, phosphoribosylglycinamide formyltransferase 2 [EC: 2.1.2.2] (inferred from 91% identity to rme:Rmet_1820)

MetaCyc: 59% identical to phosphoribosylglycinamide formyltransferase 2 (Escherichia coli K-12 substr. MG1655)
GARTRANSFORMYL2-RXN [EC: 6.3.1.21]; Acetate kinase. [EC: 6.3.1.21, 2.7.2.1, 2.7.2.15]

Predicted SEED Role

"Phosphoribosylglycinamide formyltransferase 2 (EC 2.1.2.-)" in subsystem De Novo Purine Biosynthesis (EC 2.1.2.-)

MetaCyc Pathways

• superpathway of histidine, purine, and pyrimidine biosynthesis (44/46 steps found)
• superpathway of purine nucleotides de novo biosynthesis I (21/21 steps found)
• superpathway of purine nucleotides de novo biosynthesis II (24/26 steps found)
• superpathway of tetrahydrofolate biosynthesis and salvage (11/12 steps found)
• Bifidobacterium shunt (13/15 steps found)
• superpathway of L-threonine metabolism (15/18 steps found)
• superpathway of 5-aminoimidazole ribonucleotide biosynthesis (6/6 steps found)
• 5-aminoimidazole ribonucleotide biosynthesis I (5/5 steps found)
• 5-aminoimidazole ribonucleotide biosynthesis II (5/5 steps found)
• ethanolamine utilization (5/5 steps found)
• hexitol fermentation to lactate, formate, ethanol and acetate (15/19 steps found)
• pyruvate fermentation to acetate and (S)-lactate I (4/4 steps found)
• pyruvate fermentation to acetate II (3/3 steps found)
• superpathway of acetate utilization and formation (3/3 steps found)
• L-threonine degradation I (5/6 steps found)
• acetate and ATP formation from acetyl-CoA I (2/2 steps found)
• tetrahydrofolate salvage from 5,10-methenyltetrahydrofolate (2/2 steps found)
• mixed acid fermentation (12/16 steps found)
• (S)-propane-1,2-diol degradation (4/5 steps found)
• acetylene degradation (anaerobic) (4/5 steps found)
• pyruvate fermentation to acetate and lactate II (3/4 steps found)
• acetyl-CoA fermentation to butanoate (5/7 steps found)
• pyruvate fermentation to acetate I (2/3 steps found)
• pyruvate fermentation to acetate IV (2/3 steps found)
• pyruvate fermentation to acetate VII (2/3 steps found)
• glycine degradation (reductive Stickland reaction) (1/2 steps found)
• superpathway of Clostridium acetobutylicum acidogenic fermentation (6/9 steps found)
• purine nucleobases degradation II (anaerobic) (16/24 steps found)
• reductive glycine pathway of autotrophic CO₂ fixation (5/9 steps found)
• superpathway of L-alanine fermentation (Stickland reaction) (5/9 steps found)
• superpathway of N-acetylneuraminate degradation (14/22 steps found)
• superpathway of Clostridium acetobutylicum acidogenic and solventogenic fermentation (10/17 steps found)
• methanogenesis from acetate (2/6 steps found)
• (S)-lactate fermentation to propanoate, acetate and hydrogen (7/13 steps found)
• superpathway of fermentation (Chlamydomonas reinhardtii) (4/9 steps found)
• gallate degradation III (anaerobic) (5/11 steps found)
• lactate fermentation to acetate, CO₂ and hydrogen (Desulfovibrionales) (2/8 steps found)
• L-lysine fermentation to acetate and butanoate (3/10 steps found)
• purine nucleobases degradation I (anaerobic) (6/15 steps found)
• superpathway of methanogenesis (2/21 steps found)
• superpathway of L-lysine degradation (12/43 steps found)

KEGG Metabolic Maps

• Nucleotide sugars metabolism
• One carbon pool by folate
• Propanoate metabolism
• Purine metabolism
• Pyruvate metabolism
• Taurine and hypotaurine metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.1.2.2, 2.7.2.1

Use Curated BLAST to search for 2.1.2.- or 2.1.2.2 or 2.7.2.1 or 2.7.2.15 or 6.3.1.21

Sequence Analysis Tools

See A0A0C4YGC6 at UniProt or InterPro

Protein Sequence (386 amino acids)

>RR42_RS22745 phosphoribosylglycinamide formyltransferase (Cupriavidus basilensis FW507-4G11)
MLLGSGELGKEVLIALQRLGVETIAVDRYADAPGQQVAHHARTISMSDPDQLKALIEAEK
PDLVVPEIEAIATQMLETLEAAGVVRVIPTARAARLTMDREGIRRLAAETLGVPTSPYKF
CDSLEELQAAIDGGIGYPCVVKPVMSSSGKGQSKIDGPADVKAAWDYAMAGGRVSHGRVI
VEGFIDFDYEITLLTVRAIGADGQVETQFCEPIGHVQVSGDYVESWQPHPMHPAALQKSQ
QIAQAVTANLGGQGLFGVELFVKGEQVWFSEVSPRPHDTGMVTMITQWQNEFELHARAIL
GLPVSTALKSPGASAVIYGGVDAKGVVFDGVDEALRVPQTELRLFGKPESFTKRRMGVAL
AYDADVEAARVRAKDAAGRVKPRVAG