Protein Info for RR42_RS22480 in Cupriavidus basilensis FW507-4G11

Annotation: FAD-dependent oxidoreductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 577 PF01494: FAD_binding_3" amino acids 28 to 371 (344 residues), 209.1 bits, see alignment E=1.1e-65

Best Hits

KEGG orthology group: K05712, 3-(3-hydroxy-phenyl)propionate hydroxylase [EC: 1.14.13.-] (inferred from 75% identity to reu:Reut_B4218)

Predicted SEED Role

"Salicylate hydroxylase (EC 1.14.13.1)" in subsystem Salicylate and gentisate catabolism or Salicylate ester degradation (EC 1.14.13.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.14.13.-, 1.14.13.1

Use Curated BLAST to search for 1.14.13.- or 1.14.13.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0C4Y906 at UniProt or InterPro

Protein Sequence (577 amino acids)

>RR42_RS22480 FAD-dependent oxidoreductase (Cupriavidus basilensis FW507-4G11)
MSNDYQNQSFEYQPCREQRQAHAGKPHPVIVVGAGPVGLATAIDLAQHGVPVVLVDDDCT
LSSGSRAICFAKRTLDVFDRLGCGEPMVDKGVRWHVGKVFVRDEQVYTFDLLPEAGHQRP
AFINLQQYYVEGFLLDRARQLPNLEIRWKHKVTGIEQRHAGTPDAAVVLSVDTPDGPYAL
TGRYVVAADGSRSPLRNLLGLDSKGRTFKDRFLIADVKMEAEFPTERWFWFDPPFHPNQS
VLLHRQPDNVWRIDFQLGWDADPVLEKTPERVIPRVQALLGPNAKFELEWVSVYTFSCLR
MDSFRHGHVLFAGDSAHGVSPFGARGANSGVQDAENLAWKLAYVIQGRAPDSLLDTYASE
REYAADENIRNSTRSTDFITPKSAVSKVFRDAVLALSKKHAFARQLVNSGRLSLPSVLRD
STLNTPDTDDFAAAMEPGTACVDAPVRGAQGQGWLLPQLGGQFTALLFGTPDADSLAALQ
RLQQGTLPLRLVVVSADAMPAASPGIAVLHDAEGLAAQRYDARPGTLYLIRPDQHVCARW
RQVDIAAVQQAMSRATGNLPGTQTSAPAAQAAAPVAA