Protein Info for RR42_RS21430 in Cupriavidus basilensis FW507-4G11

Annotation: alpha-ketoglutarate permease

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 436 transmembrane" amino acids 23 to 51 (29 residues), see Phobius details amino acids 63 to 85 (23 residues), see Phobius details amino acids 97 to 115 (19 residues), see Phobius details amino acids 121 to 142 (22 residues), see Phobius details amino acids 164 to 184 (21 residues), see Phobius details amino acids 196 to 215 (20 residues), see Phobius details amino acids 245 to 264 (20 residues), see Phobius details amino acids 284 to 303 (20 residues), see Phobius details amino acids 314 to 333 (20 residues), see Phobius details amino acids 339 to 362 (24 residues), see Phobius details amino acids 373 to 395 (23 residues), see Phobius details amino acids 407 to 425 (19 residues), see Phobius details PF00083: Sugar_tr" amino acids 24 to 222 (199 residues), 97.2 bits, see alignment E=1.6e-31 amino acids 230 to 426 (197 residues), 39 bits, see alignment E=7.6e-14 TIGR00883: MFS transporter, metabolite:H+ symporter (MHS) family protein" amino acids 31 to 420 (390 residues), 467.8 bits, see alignment E=1.6e-144 PF07690: MFS_1" amino acids 57 to 280 (224 residues), 61.7 bits, see alignment E=8.9e-21 amino acids 272 to 421 (150 residues), 43.4 bits, see alignment E=3.2e-15

Best Hits

Swiss-Prot: 68% identical to KGTP_SHIFL: Alpha-ketoglutarate permease (kgtP) from Shigella flexneri

KEGG orthology group: K03761, MFS transporter, MHS family, alpha-ketoglutarate permease (inferred from 86% identity to reu:Reut_B5495)

MetaCyc: 68% identical to alpha-ketoglutarate:H+ symporter (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-23

Predicted SEED Role

"dicarboxylic acid transporter PcaT" in subsystem Protocatechuate branch of beta-ketoadipate pathway

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0C4YIQ8 at UniProt or InterPro

Protein Sequence (436 amino acids)

>RR42_RS21430 alpha-ketoglutarate permease (Cupriavidus basilensis FW507-4G11)
MTDLSAPLLDAPPSAEEKRKRVYAIVAASSGNLVEWFDFYVYAFCAIYFAGSFFPKSDPT
AQLLNTAGVFAAGFLMRPIGGWLFGRIADRHGRKNSLLISVTMMCCGSLLIASLPTYNSI
GAWAPALLLVARLLQGLSVGGEYGTTATYMSEVALKGQRGFFSSFQYVTLIGGQLLAVLV
VVVLQQFLDEAELKAWGWRIPFVIGAITAVVALFLRRTLHETTSAATRASKEAGTLTELF
RNHKAAFFTVLGYTAGGSLIFYTFTTYMQKYLVNSAGMSIKTASYVMTGCLFFYMCMQPF
FGALSDRIGRRTNMLLFGALGTLATVPILTALGSVKSPFVAFALISLALAIVSLYTSISG
IVKAEMFPTEIRALGVGLAYAVANAIFGGSAEYVALDLKSLGHESSFYWYVTAMMVIVFL
VSFRLPRQARYLHHEH