Protein Info for RR42_RS20055 in Cupriavidus basilensis FW507-4G11

Annotation: ATP-dependent DNA helicase Rep

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 701 PF00580: UvrD-helicase" amino acids 5 to 277 (273 residues), 285.5 bits, see alignment E=1.6e-88 PF13245: AAA_19" amino acids 9 to 260 (252 residues), 82.1 bits, see alignment E=1.3e-26 PF13361: UvrD_C" amino acids 282 to 432 (151 residues), 130.4 bits, see alignment E=3.3e-41 amino acids 508 to 643 (136 residues), 97.7 bits, see alignment E=3e-31 PF13538: UvrD_C_2" amino acids 578 to 638 (61 residues), 32.3 bits, see alignment 2.2e-11

Best Hits

KEGG orthology group: K03656, ATP-dependent DNA helicase Rep [EC: 3.6.4.12] (inferred from 93% identity to reh:H16_A3617)

Predicted SEED Role

"ATP-dependent DNA helicase Rep" in subsystem DNA repair, bacterial UvrD and related helicases

Isozymes

Compare fitness of predicted isozymes for: 3.6.4.12

Use Curated BLAST to search for 3.6.4.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0C4YEZ3 at UniProt or InterPro

Protein Sequence (701 amino acids)

>RR42_RS20055 ATP-dependent DNA helicase Rep (Cupriavidus basilensis FW507-4G11)
MTHGLNPAQSEAVRYLDGPCLVLAGAGSGKTRVITQKIAHLIEDKGFQPKHIAAVTFTNK
AAKEMQERIAKLMEGKTTREGKRIPIKQITVSTFHSLGVQILRAEAEHVGLKPRFSIMDS
DDCFGMVQEQLASTDKKLIRSVQSTISLWKNGMVDPETAIARADNPDDHQAALIYRNYVA
TLHAYQAVDFDDLIRLPAELFARNEEVRLRWQNRLRYFLVDEYQDTNACQYQLLKQLAGG
SHLRAPAFTAVGDDDQAIYGWRGATLDNLKLLQTDFPDLKVVKLEQNYRSTVRILEAANA
VISNNPKLFDKKLWSEHGMGDPIAVNPMNDEEHEAESVVFRLSAHKFERRAQFRDYAILY
RGNHQARLFEQILRRERIPYVLSGGQSFFDKAEIKDICAYLRLIANPDDDPAFIRAITTP
KRGVGNTTLEVLGTFAGQAKVSLFEAAMMGGIEGKLQPRQLEPLRVFCEAMVRLAGRAAS
DPATQVLDDMMEGIHYEAYLYDTFDERQAQSRWTNTLEFLDWLKRKGTKPEATGEGEEAT
GFDTADGFGDEGKNLLELTQTVALMSMLEGREEDPDAVRLSTLHASKGLEYPHVFLVGVE
EGILPHCREDEDMSDEKIEEERRLMYVGITRAQRSLHLSWCKKRKRARDTYSCEPSRFIG
EMKLEEAPAIKDETPAMSPKDRLAGLKALLGTPGTPGKTSA