Protein Info for RR42_RS19860 in Cupriavidus basilensis FW507-4G11

Annotation: tartronate semialdehyde reductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 308 signal peptide" amino acids 1 to 26 (26 residues), see Phobius details PF02826: 2-Hacid_dh_C" amino acids 3 to 78 (76 residues), 33.5 bits, see alignment E=7.2e-12 PF07991: IlvN" amino acids 4 to 88 (85 residues), 22.2 bits, see alignment E=2.3e-08 PF03446: NAD_binding_2" amino acids 6 to 171 (166 residues), 165.5 bits, see alignment E=2.7e-52 TIGR01505: 2-hydroxy-3-oxopropionate reductase" amino acids 7 to 302 (296 residues), 410.3 bits, see alignment E=2.1e-127 PF03807: F420_oxidored" amino acids 7 to 76 (70 residues), 31 bits, see alignment E=8.2e-11 PF14833: NAD_binding_11" amino acids 174 to 293 (120 residues), 139.7 bits, see alignment E=1.4e-44

Best Hits

Swiss-Prot: 68% identical to GLXR_ECOLI: 2-hydroxy-3-oxopropionate reductase (glxR) from Escherichia coli (strain K12)

KEGG orthology group: K00042, 2-hydroxy-3-oxopropionate reductase [EC: 1.1.1.60] (inferred from 95% identity to reh:H16_A3600)

MetaCyc: 68% identical to tartronate semialdehyde reductase 2 (Escherichia coli K-12 substr. MG1655)
1.1.1.60,4.1.1.M27,4.1.1.73 [EC: 1.1.1.60, 4.1.1.73, 4.1.1.M27]

Predicted SEED Role

"2-hydroxy-3-oxopropionate reductase (EC 1.1.1.60)" in subsystem Allantoin Utilization or D-galactarate, D-glucarate and D-glycerate catabolism or Photorespiration (oxidative C2 cycle) (EC 1.1.1.60)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.60, 4.1.1.73

Use Curated BLAST to search for 1.1.1.60 or 4.1.1.73 or 4.1.1.M27

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0C4YL76 at UniProt or InterPro

Protein Sequence (308 amino acids)

>RR42_RS19860 tartronate semialdehyde reductase (Cupriavidus basilensis FW507-4G11)
MANQRNVGFIGLGIMGAPMAGHLRAAGHTLFVHDVNPAPQALVDAGVTVCTSAEEVAKRA
DIIVIMVPDTPHVEAVLFAEKGVAAALKGAGKQAAHGKIVVDMSSISPIATKDFAARVNK
LGAAYLDAPVSGGEVGAKAASLTIMVGGPQESFDEVKPLFDLLGKNVTLVGGNGDGQTTK
VANQIIVALNIQAVSEALLFASKAGADPARVRQALMGGFAASRILEVHGERMVKRTFDPG
FRIELHQKDLNLALQGAKALGVALPNTATAQELFNTCAANGLGKQDHSALCRAIEIMSNH
EIAKGGAK