Protein Info for RR42_RS19310 in Cupriavidus basilensis FW507-4G11

Annotation: ATP-dependent DNA helicase RecQ

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 621 TIGR01389: ATP-dependent DNA helicase RecQ" amino acids 6 to 614 (609 residues), 786.6 bits, see alignment E=1.4e-240 TIGR00614: ATP-dependent DNA helicase, RecQ family" amino acids 7 to 468 (462 residues), 500.6 bits, see alignment E=4.6e-154 PF00270: DEAD" amino acids 21 to 181 (161 residues), 90.2 bits, see alignment E=3.3e-29 PF00271: Helicase_C" amino acids 235 to 332 (98 residues), 65.5 bits, see alignment E=1.2e-21 PF16124: RecQ_Zn_bind" amino acids 344 to 405 (62 residues), 67.9 bits, see alignment E=2.6e-22 PF09382: RQC" amino acids 407 to 523 (117 residues), 133.1 bits, see alignment E=9.8e-43 PF00570: HRDC" amino acids 550 to 615 (66 residues), 82.7 bits, see alignment E=3.7e-27

Best Hits

KEGG orthology group: K03654, ATP-dependent DNA helicase RecQ [EC: 3.6.4.12] (inferred from 88% identity to cti:RALTA_A2956)

Predicted SEED Role

"ATP-dependent DNA helicase RecQ" in subsystem DNA-replication or DNA repair, bacterial RecFOR pathway

Isozymes

Compare fitness of predicted isozymes for: 3.6.4.12

Use Curated BLAST to search for 3.6.4.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0C4YKW4 at UniProt or InterPro

Protein Sequence (621 amino acids)

>RR42_RS19310 ATP-dependent DNA helicase RecQ (Cupriavidus basilensis FW507-4G11)
MSRALAILKDVFGYHAFRGRQGEIIDHVADGGDCLVLMPTGGGKSLCYQIPALLRQRAGQ
GTGIVVSPLIALMQDQVAALSEAGVRAAVLNSTLSGAEASAVERDLLAGRLEILYVAPER
LMTPRFQDLLQRTRIGVFAIDEAHCVSQWGHDFRPEYIQLSVLHERFPEVPRIALTATAD
SVTREEIVERLALHDARVFVSSFDRPNIRYRIVEKDNARQQLLAFIKAEHLAADGTHDSG
IVYCLSRKKVEDTASYLSAQGLNALAYHAGMDAGVRQHHQARFREEEGVIMVATIAFGMG
IDKPDVRFVAHLDLPKSMEGYYQETGRAGRDGLPANAWMAYGLGDVVQQKRMIDESEADD
AHKRVSSAKLDALLGLCETAGCRRVRILGYFDEASEPCGNCDTCLEPPATWDGTREAQMA
LSCVYRTAQASRVHFGATHMVDVLRGNASEKIRQWGHDKVSTFGIGADRSVSEWHAIFRQ
LIAHGLLVIDHGGHGALLLGDAARAVLKGEMPITLRRQASKPAKSGERGVRGSAGTRTDH
TAGMDTEALANWEALRKWRTETAREHGVPAYVIFHDATLAELARSAPDSLTAMAEVPGIG
AAKLERYGDAILEALGAVEDF