Protein Info for RR42_RS19275 in Cupriavidus basilensis FW507-4G11

Annotation: carboxymethylenebutenolidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01738: DLH
  • amino acids 14 to 227 (214 residues), 158.1 bits, E=1.3e-50

Best Hits

KEGG orthology group: K01061, carboxymethylenebutenolidase [EC: 3.1.1.45] (inferred from 89% identity to reh:H16_A3488)

Predicted SEED Role

"Dienelactone hydrolase family"

MetaCyc Pathways

• 3-chlorocatechol degradation II (ortho) (3/5 steps found)
• 4-chlorocatechol degradation (3/5 steps found)
• 4,5-dichlorocatechol degradation (4/7 steps found)
• 3,4,6-trichlorocatechol degradation (5/9 steps found)
• 3-chlorocatechol degradation I (ortho) (2/5 steps found)
• 3,5-dichlorocatechol degradation (4/8 steps found)
• 1,4-dichlorobenzene degradation (4/9 steps found)

KEGG Metabolic Maps

• 1,4-Dichlorobenzene degradation
• Fluorobenzoate degradation
• gamma-Hexachlorocyclohexane degradation

Isozymes

Compare fitness of predicted isozymes for: 3.1.1.45

Use Curated BLAST to search for 3.1.1.45

Sequence Analysis Tools

See A0A0C4YEM1 at UniProt or InterPro

Protein Sequence (409 amino acids)

>RR42_RS19275 carboxymethylenebutenolidase (Cupriavidus basilensis FW507-4G11)
MGQTIQIHAADGAFNAYLATPASGKGPGIVLCQEIFGVNATMRAVADYYAEEGYTVLVPD
LFWRIAPGIELGDRGEDFQRALGLYQRFDEALGVKDVGAALDALRARPECVGQTGVLGFC
LGGKLAYLAACRLDGVACAVAYYGVGIERALDEAAKIQGRLVLHVAERDGFCPPEAQAQI
RAALGGKDGIELYVYPGVDHAFARTGGEHFDHPSALMAHQRSIAALRAEMGPHYDFSALW
DKHCEYEFATRDVDATMATMVSEPYVNHIPTMTGGVGYRQLKRFYQNHFVNSNPPDTSLI
PLSRTVGASQIVDEILFCFTHTCEVDWMLPGVAPTGKRVEIPLIAIVKFRGDKLYHEHIY
WDQASVLVQIGKLDPAGLPVAGVETARKLLDETLPSNTLMARWQESEGK