Protein Info for RR42_RS18685 in Cupriavidus basilensis FW507-4G11

Annotation: restriction endonuclease subunit M

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 834 PF12161: HsdM_N" amino acids 8 to 150 (143 residues), 96 bits, see alignment E=3.2e-31 PF02384: N6_Mtase" amino acids 164 to 488 (325 residues), 322.7 bits, see alignment E=2.8e-100

Best Hits

KEGG orthology group: K03427, type I restriction enzyme M protein [EC: 2.1.1.72] (inferred from 84% identity to reh:H16_A0004)

Predicted SEED Role

"Type I restriction-modification system, DNA-methyltransferase subunit M (EC 2.1.1.72)" in subsystem Restriction-Modification System (EC 2.1.1.72)

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.72

Use Curated BLAST to search for 2.1.1.72

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0C4YKM8 at UniProt or InterPro

Protein Sequence (834 amino acids)

>RR42_RS18685 restriction endonuclease subunit M (Cupriavidus basilensis FW507-4G11)
MPLTLPQLERHLFKAADILRGKMDASEFKEYIFGMLFLKRCSDVFEQRRAEVIQLEIDAG
KTPAAAEASAENKRWYKKDGYFWVPKESRYSYLVDNSRQANVGGLLSKALAGIEGDNVSL
YDVLEHIDFTRKIGQSKIPDLKLQQLITHFSLHRLRNEDFEFPDLLGAAYEYLIGEFADS
AGKKGGEFYTPRSVVRMMVRLIKPELKHDIYDPCCGSGGMLIAAKEYIDEHGEDGRKANL
FGQEFNGTVWSIAKMNMLLHGISTANLQNEDTLAEPQHVEGGELMHFDRVLTNPPFSIHW
GNTEKDADGKPAWKPKFEAERFPYGQVPLGAKKADLMFLQHMLAVTRDGGMVATVLPHGV
LFRGGEEKAIRAGIIEDDLLEAVIGVAPNLFYGTGIPACILVLRQRLQNGANRVSGKPAE
RQGKVLFINADREFFEGRAQNYLLPEHIEKIVTTFDEFRAIDGFSAIVDNSTLKANDYNL
NIRRYADNAPPPEPHDVRAHLVGGIPKAEVANKASLFSAHGLNPMDLLVERDAKYYDFAP
KLDKRQALKPTIESNAGLVAKEAAIRAAFEQWWQDHSARITALSGQMDNAASLVALRNDL
LASFSQSLEAIGLLDPFQVRGIVAGFWYQTKYDFLTLMARGAKGVADAWRTSIVTALEDK
ASKESPLEHKLVKFLMSDFVEAITELEAKKAELDSQIKAASPKDGEGEDGEGAETVEDDA
DEENAVDEVQLKAWKKELAAVKKQLKAKNDSFKQHIGAAVEGLTPEAAAELLLTILHNDM
QAIVEQYMAAQRKQIVGAFENWWDKYRVTLTEIEGKRDGAAKALQGFLKDIGYV