Protein Info for RR42_RS17275 in Cupriavidus basilensis FW507-4G11

Annotation: molecular chaperone DnaK

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 654 TIGR02350: chaperone protein DnaK" amino acids 3 to 604 (602 residues), 957.5 bits, see alignment E=1.3e-292 PF00012: HSP70" amino acids 4 to 604 (601 residues), 881.6 bits, see alignment E=2.4e-269 PF06723: MreB_Mbl" amino acids 114 to 378 (265 residues), 42.4 bits, see alignment E=4.3e-15

Best Hits

Swiss-Prot: 95% identical to DNAK_CUPNH: Chaperone protein DnaK (dnaK) from Cupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337)

KEGG orthology group: K04043, molecular chaperone DnaK (inferred from 94% identity to cti:RALTA_A2564)

MetaCyc: 73% identical to chaperone protein DnaK (Escherichia coli K-12 substr. MG1655)
Non-chaperonin molecular chaperone ATPase. [EC: 3.6.4.10, 5.6.1.7]

Predicted SEED Role

"Chaperone protein DnaK" in subsystem Heat shock dnaK gene cluster extended or Protein chaperones

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.6.4.10 or 5.6.1.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0C4Y637 at UniProt or InterPro

Protein Sequence (654 amino acids)

>RR42_RS17275 molecular chaperone DnaK (Cupriavidus basilensis FW507-4G11)
MGKIIGIDLGTTNSCVAIMEGNTPKVIENAEGTRTTPSIIAYMEDGEILVGAPAKRQAVT
NPRNTLYAVKRLIGRKFEEKEVQKDIGLMPYTIVKADNGDAWVSVRDQKLAPPQVSAEVL
RKMKKTAEDYLGEPVTEAVITVPAYFNDSQRQATKDAGRIAGLDVKRIINEPTAAALAFG
LDKNEKGDRKIAVYDLGGGTFDISIIEIADVDGEKQFEVLSTNGDTFLGGEDFDQRIIDY
IIGEFKKDQGVDLSKDVLALQRLKEAAEKAKIELSSSQQTEINLPYITADASGPKHLNLK
MTRAKLESLVEELILRTIEPCRIAIKDAGVKVSEIDDVILVGGMTRMPKVQEQVKEFFGK
EARKDVNPDEAVAVGAAIQGSVLSGDRKDLLLLDVTPLSLGIETLGGVMTKMITKNTTIP
TKHAQVFSTADDNQPAVTIKVFQGEREMATGNKLLGEFNLEGIAPAARGTPQIEVSFDID
ANGILHVGAKDKATGKENRITIKANSGLSEDEIQRMVKDAEANAEEDKKARELADARNQA
DALIHSTKKAVTEYGDKLEAGEKEKIEAAIKELEDAARGGDKAEIDAKVSALSEASQKLG
ERVYADMQAQAGEAGAAGAAAGAGAGAAGAGHQQAHPQDDNVVDAEFKEVNDKK