Protein Info for RR42_RS16250 in Cupriavidus basilensis FW507-4G11

Annotation: sulfate adenylyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 434 TIGR02034: sulfate adenylyltransferase, large subunit" amino acids 11 to 430 (420 residues), 530.8 bits, see alignment E=1.2e-163 PF00009: GTP_EFTU" amino acids 11 to 209 (199 residues), 137.8 bits, see alignment E=3.3e-44

Best Hits

Swiss-Prot: 50% identical to CYSN_CHRSD: Sulfate adenylyltransferase subunit 1 (cysN) from Chromohalobacter salexigens (strain DSM 3043 / ATCC BAA-138 / NCIMB 13768)

KEGG orthology group: K00956, sulfate adenylyltransferase subunit 1 [EC: 2.7.7.4] (inferred from 91% identity to reu:Reut_A2692)

MetaCyc: 51% identical to sulfate adenylyltransferase large subunit (Allochromatium vinosum)
Sulfate adenylyltransferase. [EC: 2.7.7.4]

Predicted SEED Role

"Sulfate adenylyltransferase subunit 1 (EC 2.7.7.4)" in subsystem Cysteine Biosynthesis (EC 2.7.7.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.4

Use Curated BLAST to search for 2.7.7.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0C4YFF7 at UniProt or InterPro

Protein Sequence (434 amino acids)

>RR42_RS16250 sulfate adenylyltransferase (Cupriavidus basilensis FW507-4G11)
MSTQALHQGLLRFITAGSVDDGKSTLIGRLLYDSKAVLSDQLTALTNAKNKRTAGEQIDF
SLLTDGLEAEREQGITIDVAYRYFSTARRKFIIADTPGHEQYTRNMVTGASTAHAAIVLV
DATRVTVTDGRAELLAQTKRHSAILKLLELQHVIVAINKMDLVEFSEERFNQIRAAYTEL
ASQLGLKDVRYVPVSALRGDNIVNASQAMPWYQGEPLLPLLEALPVEEPAPEGDAALRFP
VQLVIRQDGAQADDFRGYAGRVEAGTVRVGQKLRVLPANRDALVAEVLTPNGVAESASVG
ETVTVRLSEDVDVSRGDMFVAAAADSVSAKKLTADLCWFDDESLNPARKYVLKHTTASVF
ARVSSVDRVLDVHTLLHETDRHEIGLNDIGSVQISLQKPIVCDNYGDNPATGAFVLIDEA
TNHTVAAGMIRAFA