Protein Info for RR42_RS15660 in Cupriavidus basilensis FW507-4G11

Annotation: protein-L-isoaspartate O-methyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 217 PF01135: PCMT" amino acids 10 to 206 (197 residues), 125.6 bits, see alignment E=1.2e-39 PF00398: RrnaAD" amino acids 63 to 135 (73 residues), 32.2 bits, see alignment E=3e-11 PF13489: Methyltransf_23" amino acids 68 to 158 (91 residues), 31.4 bits, see alignment E=7.7e-11 PF05175: MTS" amino acids 69 to 147 (79 residues), 26.1 bits, see alignment E=3e-09 PF01209: Ubie_methyltran" amino acids 71 to 182 (112 residues), 22.2 bits, see alignment E=4e-08 PF06325: PrmA" amino acids 74 to 194 (121 residues), 22.2 bits, see alignment E=4.5e-08 PF13847: Methyltransf_31" amino acids 77 to 171 (95 residues), 37.7 bits, see alignment E=8.9e-13 PF13649: Methyltransf_25" amino acids 81 to 158 (78 residues), 44.7 bits, see alignment E=9.3e-15 PF08241: Methyltransf_11" amino acids 82 to 156 (75 residues), 33.6 bits, see alignment E=2.5e-11

Best Hits

Swiss-Prot: 38% identical to PIMT2_MARHV: Protein-L-isoaspartate O-methyltransferase 2 (pcm2) from Marinobacter hydrocarbonoclasticus (strain ATCC 700491 / DSM 11845 / VT8)

KEGG orthology group: K00573, protein-L-isoaspartate(D-aspartate) O-methyltransferase [EC: 2.1.1.77] (inferred from 84% identity to reh:H16_A2877)

MetaCyc: 35% identical to protein-L-isoaspartate O-methyltransferase (Escherichia coli K-12 substr. MG1655)
Protein-L-isoaspartate(D-aspartate) O-methyltransferase. [EC: 2.1.1.77]

Predicted SEED Role

"Protein-L-isoaspartate O-methyltransferase (EC 2.1.1.77)" in subsystem Ton and Tol transport systems (EC 2.1.1.77)

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.77

Use Curated BLAST to search for 2.1.1.77

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0C4YEX5 at UniProt or InterPro

Protein Sequence (217 amino acids)

>RR42_RS15660 protein-L-isoaspartate O-methyltransferase (Cupriavidus basilensis FW507-4G11)
MDLEKARFNMIEQQIRPWDVLDQEILDLLAVVKREQFVPQAYAALAFVDMEIPLPGGQNM
LAPRVEARILQDLAVRKHEQVLEIGAGSGYMAALLAHRARHVLSVDILPELAELARKNLA
HAGVTNVDVAQGNAATGWAASAPYDVICISGALPSVPPSILSQVKVGGRIAAFVGALPAM
EAQIITRVSENEYKTVNLFETAITPLMGAEQPSRFHF