Protein Info for RR42_RS15095 in Cupriavidus basilensis FW507-4G11

Annotation: valyl-tRNA synthetase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 955 transmembrane" amino acids 500 to 518 (19 residues), see Phobius details TIGR00422: valine--tRNA ligase" amino acids 9 to 935 (927 residues), 1069.9 bits, see alignment E=0 PF00133: tRNA-synt_1" amino acids 24 to 626 (603 residues), 463.3 bits, see alignment E=1.8e-142 PF09334: tRNA-synt_1g" amino acids 48 to 192 (145 residues), 29.7 bits, see alignment E=7.5e-11 PF13603: tRNA-synt_1_2" amino acids 251 to 306 (56 residues), 26.7 bits, see alignment 9.1e-10 PF08264: Anticodon_1" amino acids 677 to 830 (154 residues), 132.7 bits, see alignment E=2.5e-42 PF10458: Val_tRNA-synt_C" amino acids 889 to 953 (65 residues), 67.9 bits, see alignment 1.9e-22

Best Hits

Swiss-Prot: 91% identical to SYV_CUPNJ: Valine--tRNA ligase (valS) from Cupriavidus necator (strain JMP 134 / LMG 1197)

KEGG orthology group: K01873, valyl-tRNA synthetase [EC: 6.1.1.9] (inferred from 75% identity to bcn:Bcen_0959)

Predicted SEED Role

"Valyl-tRNA synthetase (EC 6.1.1.9)" (EC 6.1.1.9)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0C4YI61 at UniProt or InterPro

Protein Sequence (955 amino acids)

>RR42_RS15095 valyl-tRNA synthetase (Cupriavidus basilensis FW507-4G11)
MTAQDQSLAKSFEPAAIEAKWGPEWEKRGIAKPTLDPDRPDFCIQLPPPNVTGTLHMGHA
FNQTIMDGLARHARMRGANTLWVPGTDHAGIATQIVVERQLEAQGVSRHELGREKFVEKV
WEWKEESGSTITRQIRRMGASIDWSHEYFTMSPEMSKAVTEVFVRLFEQGLIYRGKRLVN
WDPVLGTAVSDLEVDNVEEDGSMWHIRYPLAEADTAHGLTHLTVATTRPETMLGDVAVMV
HPQDERYAHLIGKFVRLPLTGRDIPVIADDYVDREFGTGVVKVTPAHDFNDYAVGQRHKL
AQLSILTLDAKIVADAPAAYAGLDRFEARTRIVADLDAQGLLAEVKKHKLMTPRSERTGS
AIEPMLTDQWFVAMSKPAPEGTYHPGRSIAEVALDAVQSGEIKLIPDNWNSTYNQWLENI
QDWCISRQLWWGHQIPAWYDEAGKCFVARTEEEAQAQARAAGSTGPLRREDDVLDTWFSS
ALVPFSSLGWPAETPALQHFLPSSALVTGYDIIFFWVARMVMMTKHFTGKVPFHTVYVHG
LVRDSEGKKMSKSEGNTLDPVDLIDGIELEPLLKKRTTGLRRPKDAPKIEKKTKKEFPEG
IPAFGADALRFTFASLATLGRNINFDTGRCEGYRNFCNKLWNATRFVLMNTEGHDCGMGP
CSNDCGPDGYLHFSQPDRWIVSLLQRVEAEVEKGFAEYRFDNIASAIYKFVWDEYCDWYL
ELAKVQIQNGTPAQQRATRRTLLRVLETVLRLAHPIIPFITEELWQKVAPLAGRAKGDGS
ETLALQAYPLPAMAKIDEAAEQWTAQLKAVVDACRNLRGEMNISPAQRIPLYAEGDSAFL
TSAQAYVQALAKLSEVKVFEDQNALMAEGAGAPVAIVGGNHLLLKIEIDVAAERVRLSKE
IERISAELGKCRGKLSNESFVAKAPPAVVAQETQRLSDFEQTLVKLQDQLQRLPA