Protein Info for RR42_RS15040 in Cupriavidus basilensis FW507-4G11

Annotation: carboxymethylenebutenolidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 58% identical to DLHH_METEA: Putative carboxymethylenebutenolidase (MexAM1_META1p1735) from Methylobacterium extorquens (strain ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1)

KEGG orthology group: K01061, carboxymethylenebutenolidase [EC: 3.1.1.45] (inferred from 85% identity to reh:H16_A2739)

Predicted SEED Role

"Dienelactone hydrolase family"

MetaCyc Pathways

3-chlorocatechol degradation II (ortho) (3/5 steps found)
4-chlorocatechol degradation (3/5 steps found)
4,5-dichlorocatechol degradation (4/7 steps found)
3,4,6-trichlorocatechol degradation (5/9 steps found)
3-chlorocatechol degradation I (ortho) (2/5 steps found)
3,5-dichlorocatechol degradation (4/8 steps found)
1,4-dichlorobenzene degradation (4/9 steps found)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.1.1.45

Use Curated BLAST to search for 3.1.1.45

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0C4YBX2 at UniProt or InterPro

Protein Sequence (290 amino acids)

>RR42_RS15040 carboxymethylenebutenolidase (Cupriavidus basilensis FW507-4G11)
MLKPEVESLVPSQSFDRRAFVKTALGSAFAAAALPVMAQAIKTDFAGLAAGEVTIPSGGF
NMPAYRAQPEGKTNLPVVLVVSEIFGVHEHIADICRRFAKLGYLAIAPELFARQGDPSSF
GTIQELQAKLISKVPDEQVMGDLDAAVAWAKGNGGDTSRLAITGFCWGGRITWLYAAHSQ
QIKAGVAWYGQLVGEPTPIKPRNPIDLAGQLKVPVLGLYGGKDTGITQEQVEKMKAALAV
SGDPFAKASTFVVYPESGHAFNADYRPSYREADAKDGWQRCLAWFKQHGV