Protein Info for RR42_RS15020 in Cupriavidus basilensis FW507-4G11

Annotation: (2Fe-2S)-binding protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 367 PF00355: Rieske" amino acids 43 to 121 (79 residues), 47.3 bits, see alignment E=1.6e-16 PF00848: Ring_hydroxyl_A" amino acids 172 to 364 (193 residues), 139.1 bits, see alignment E=2e-44

Best Hits

KEGG orthology group: None (inferred from 89% identity to rme:Rmet_2618)

MetaCyc: 62% identical to putrescine 2-hydroxylase (Bordetella bronchiseptica)
1.14.13.M64 [EC: 1.14.13.M64]

Predicted SEED Role

"Phenylpropionate dioxygenase and related ring-hydroxylating dioxygenases, large terminal subunit" in subsystem Phenylpropanoid compound degradation

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.14.13.M64

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0C4YEC5 at UniProt or InterPro

Protein Sequence (367 amino acids)

>RR42_RS15020 (2Fe-2S)-binding protein (Cupriavidus basilensis FW507-4G11)
MSNLSTALKLVPADTQLPVNVYIDEALHQQELELLFKKGPGYVGHELMIPEAGDFQALAA
EDEGRILVRNADGIELMSNVCRHRQAIMLNGRGNAKNIVCPLHRWTYDLKGELLGAPHFA
ERPCVHLNRSPLQNWNGLLFEGQRDVRADLARLGVAEDLNFEGYLLDHVEIHECNYNWKT
FIEVYLEDYHVVPFHPGLGSFVSCDDLSWEFGDWHSVQTVGLHAGLKRPGSPTYQKWHEA
VLRFNNGVLPKYGAIWLTYYPNIMVEWYPNVLVVSTLHPLGPNKTRNVVEFYYPEEIALF
EREFVEAERAAYMETCIEDDEIAERMDAGRRALAKRGVSETGPYQSPMEDGMQHFHEWYR
RAMSFTD