Protein Info for RR42_RS14155 in Cupriavidus basilensis FW507-4G11

Annotation: pseudouridine synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 333 TIGR00005: pseudouridine synthase, RluA family" amino acids 29 to 325 (297 residues), 267.7 bits, see alignment E=6.5e-84 PF01479: S4" amino acids 33 to 79 (47 residues), 34.1 bits, see alignment 1.8e-12 PF00849: PseudoU_synth_2" amino acids 110 to 259 (150 residues), 104 bits, see alignment E=9.6e-34

Best Hits

Swiss-Prot: 49% identical to RLUC_PSEAE: Ribosomal large subunit pseudouridine synthase C (rluC) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K06179, ribosomal large subunit pseudouridine synthase C [EC: 5.4.99.12] (inferred from 78% identity to reh:H16_A2578)

Predicted SEED Role

"Ribosomal large subunit pseudouridine synthase C (EC 4.2.1.70)" in subsystem Ribosome biogenesis bacterial (EC 4.2.1.70)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.70, 5.4.99.12

Use Curated BLAST to search for 4.2.1.70 or 5.4.99.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0C4Y4H8 at UniProt or InterPro

Protein Sequence (333 amino acids)

>RR42_RS14155 pseudouridine synthase (Cupriavidus basilensis FW507-4G11)
MNELRHQIEKEVRPAAAGPQVAYVTIGDEADGQRIDNFLLKLSKGVPKSHVYRILRSGEV
RVNKGRVDATYRLQLGDVVRIPPMRVAASAPDAGKFVPPASFPVVFEDEYLLVINKPAGV
AVHGGSGVAFGVIEQLRRARPQARFLELVHRLDRETSGLLVLAKKRTALVNLHEQMRGSA
LDKRYFACVAGHFENARQHVKFPLYKYTTPEGERRVRVQEDGIASHTVFNRLEVMPGYTL
LEAELKTGRTHQIRVHLAHSGFPILGDEKYGDYALNKALARSGANPGLKRMFLHAHSLTF
THPISGEITTVVAPMPPECEAFLQQARNQRAAD