Protein Info for RR42_RS14055 in Cupriavidus basilensis FW507-4G11

Annotation: elongation factor 4

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00009: GTP_EFTU
  • amino acids 3 to 181 (179 residues), 183.6 bits, E=6e-58
• TIGR01393: elongation factor 4
  • amino acids 3 to 594 (592 residues), 985.9 bits, E=6.2e-301
• TIGR00231: small GTP-binding protein domain
  • amino acids 5 to 174 (170 residues), 60.9 bits, E=1.3e-20
• PF03144: GTP_EFTU_D2
  • amino acids 205 to 275 (71 residues), 46.7 bits, E=7e-16
• PF00679: EFG_C
  • amino acids 399 to 484 (86 residues), 83 bits, E=2.5e-27
• PF06421: LepA_C
  • amino acids 487 to 593 (107 residues), 166.4 bits, E=3.7e-53

Best Hits

Swiss-Prot: 93% identical to LEPA_CUPNJ: Elongation factor 4 (lepA) from Cupriavidus necator (strain JMP 134 / LMG 1197)

KEGG orthology group: K03596, GTP-binding protein LepA (inferred from 93% identity to reu:Reut_A2255)

MetaCyc: 69% identical to 30S ribosomal subunit biogenesis factor LepA (Escherichia coli K-12 substr. MG1655)
3.6.1.15,3.6.5.6,3.6.5.5,3.6.5.4,3.6.5.3,3.6.5.2,3.6.5.1,3.6.1.-,3.6.1.5 [EC: 3.6.1.15, 3.6.1.5, 3.6.5.1, 3.6.5.2, 3.6.5.3, 3.6.5.4, 3.6.5.5, 3.6.5.6]

Predicted SEED Role

"Translation elongation factor LepA" in subsystem Heat shock dnaK gene cluster extended or Universal GTPases

MetaCyc Pathways

• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (18/18 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis I (9/9 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (E. coli) (12/14 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis III (8/9 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis IV (6/7 steps found)
• pyrimidine deoxyribonucleotides biosynthesis from CTP (6/8 steps found)
• UTP and CTP dephosphorylation I (5/7 steps found)
• UTP and CTP dephosphorylation II (1/3 steps found)

KEGG Metabolic Maps

• Purine metabolism
• Pyrimidine metabolism
• Thiamine metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.6.1.15

Use Curated BLAST to search for 3.6.1.15 or 3.6.1.5 or 3.6.5.1 or 3.6.5.2 or 3.6.5.3 or 3.6.5.4 or 3.6.5.5 or 3.6.5.6

Sequence Analysis Tools

See A0A0C4YHR1 at UniProt or InterPro

Protein Sequence (597 amino acids)

>RR42_RS14055 elongation factor 4 (Cupriavidus basilensis FW507-4G11)
MDHIRNFSIIAHIDHGKSTLADRIIQLCGGLSDREMEAQVLDSMDIEKERGITIKAQTAA
LSYKARDGKIYNLNLIDTPGHVDFSYEVSRSLSACEGALLVVDASQGVEAQTVANCYTAI
ELGVEVVPVLNKIDLPSADPENAIREIEEVIGIDAHDATPCSAKTGLGVQDVIEAMIARV
PPPKGDPAAPLQALIIDSWFDNYVGVVMLVRVVNGTLKPKDKVLLMATGSQHLVEQVGVF
SPKSLPRESLQAGQVGFVIAGIKELKAAKVGDTITTMARKAAEPLPGFKEVKPQVFAGLY
PVEANQYEALRESLEKLKLNDASLQYEPEVSQALGFGFRCGFLGLLHMEIVQERLEREFD
MDLITTAPTVVYEVDMRDKTKVIVENPAKMPDPSRIENILEPIVTVNLYMPQAYVGSVIT
LCTQKRGTQINMSYHGKQVQLTYEIPMAEIVMDFFDRLKSVSRGYASMDYEFKEYRAADV
VKVDILINGDKVDALSVIVHRSNSQYRGREVVSKMREIIPRQMYDVAIQATIGVNIIARE
NVKALRKNVLAKCYGGDISRKKKLLEKQKAGKKRMKQVGSVEIPQEAFLAILQVGDK