Protein Info for RR42_RS12835 in Cupriavidus basilensis FW507-4G11

Annotation: tRNA pseudouridine synthase B

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 315 TIGR00431: tRNA pseudouridine(55) synthase" amino acids 18 to 227 (210 residues), 263.2 bits, see alignment E=8.5e-83 PF01509: TruB_N" amino acids 39 to 189 (151 residues), 187.1 bits, see alignment E=3.7e-59 PF16198: TruB_C_2" amino acids 190 to 247 (58 residues), 57.3 bits, see alignment E=2.2e-19 PF09157: TruB-C_2" amino acids 252 to 313 (62 residues), 41.1 bits, see alignment E=2.1e-14

Best Hits

Swiss-Prot: 82% identical to TRUB_CUPTR: tRNA pseudouridine synthase B (truB) from Cupriavidus taiwanensis (strain DSM 17343 / BCRC 17206 / CIP 107171 / LMG 19424 / R1)

KEGG orthology group: K03177, tRNA pseudouridine synthase B [EC: 5.4.99.12] (inferred from 82% identity to cti:RALTA_A1848)

Predicted SEED Role

"tRNA pseudouridine synthase B (EC 4.2.1.70)" in subsystem tRNA processing (EC 4.2.1.70)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.70, 5.4.99.12

Use Curated BLAST to search for 4.2.1.70 or 5.4.99.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0C4YAL9 at UniProt or InterPro

Protein Sequence (315 amino acids)

>RR42_RS12835 tRNA pseudouridine synthase B (Cupriavidus basilensis FW507-4G11)
MTHPNTNRPPRQPRREVHGVLLLDKPLGLSSNDALVRAKRMLRALKAGHTGTLDPLATGL
LPLCFGEATKFSQDLLEADKTYEATVRLGLKTATGDAEGEVIAERPVTCDVEALRAAMLR
FAGEIEQVPPMHSALKKDGRPLYEYARAGQTVERAARRVTIHEITLLDCGLPQVPTFSMR
VTCSKGTYIRTLAEDIGEALGCGAHLTGLRRTAVGDLSLEGAVTLEQIEQRSDEERPGML
APVDALLQRCVAVTLDEAAMARFLQGQRIAQRDLPAGVALAEDELASVYGGEPRRLLGVA
RMREGALRPERLVKL