Protein Info for RR42_RS10665 in Cupriavidus basilensis FW507-4G11
Annotation: branched-chain alpha-keto acid dehydrogenase subunit E2
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
KEGG orthology group: K00627, pyruvate dehydrogenase E2 component (dihydrolipoamide acetyltransferase) [EC: 2.3.1.12] (inferred from 68% identity to rpi:Rpic_4647)Predicted SEED Role
"Dihydrolipoamide acetyltransferase component (E2) of acetoin dehydrogenase complex (EC 2.3.1.-)" in subsystem Acetoin, butanediol metabolism (EC 2.3.1.-)
MetaCyc Pathways
- superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle (19/22 steps found)
- pyruvate decarboxylation to acetyl CoA I (3/3 steps found)
KEGG Metabolic Maps
- 1- and 2-Methylnaphthalene degradation
- Alkaloid biosynthesis I
- Alkaloid biosynthesis II
- Anthocyanin biosynthesis
- Benzoate degradation via CoA ligation
- Biosynthesis of alkaloids derived from histidine and purine
- Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
- Biosynthesis of alkaloids derived from shikimate pathway
- Biosynthesis of alkaloids derived from terpenoid and polyketide
- Biosynthesis of phenylpropanoids
- Biosynthesis of plant hormones
- Biosynthesis of terpenoids and steroids
- Biosynthesis of type II polyketide backbone
- Biosynthesis of unsaturated fatty acids
- Butanoate metabolism
- Carotenoid biosynthesis - General
- Citrate cycle (TCA cycle)
- Diterpenoid biosynthesis
- Ether lipid metabolism
- Ethylbenzene degradation
- Fatty acid biosynthesis
- Glycerophospholipid metabolism
- Glycolysis / Gluconeogenesis
- Glycosphingolipid biosynthesis - ganglio series
- Histidine metabolism
- Limonene and pinene degradation
- Lipopolysaccharide biosynthesis
- Lysine degradation
- Phenylalanine metabolism
- Pyruvate metabolism
- Tyrosine metabolism
Isozymes
Compare fitness of predicted isozymes for: 2.3.1.-, 2.3.1.12
Use Curated BLAST to search for 2.3.1.- or 2.3.1.12
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A0C4YFP6 at UniProt or InterPro
Protein Sequence (384 amino acids)
>RR42_RS10665 branched-chain alpha-keto acid dehydrogenase subunit E2 (Cupriavidus basilensis FW507-4G11) MIEFRLPALGADMDEGTLLEWTVKPGDPLKRGQVVAVVDTSKAAVEVECWHEGTVAELIV TPGAKIPVGTLMAVFLEPGETPGAARPATGPADAPAPSAAHGLPGIAMAPPTRHMVSPAA RKSALELGIDIDAVFATDPNGIVKLADVERAVRERAAAPVDKSAGIRKAIAVAMARSKRE IPHYYVGETIPLGATLAWLTRENANRPMAERLLPAALLIKAVAVTLKNYPELNGFFRDEV FHAVSQVHVGVAISLRQGGLIAPALLDADTKPLTLLMRELSDLVKRCRAGSLRSSEMAMP TITVTNLGDQGAEAVFGVIYPPQVALVGIGRVIERPWAENGELKVVPAVTASLSADHRVS DGHRGALFLVELRELLQHPEELNQ