Protein Info for RR42_RS08915 in Cupriavidus basilensis FW507-4G11

Annotation: nitrate reductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1100 1200 1267 TIGR01580: nitrate reductase, alpha subunit" amino acids 2 to 1256 (1255 residues), 2212.5 bits, see alignment E=0 PF14710: Nitr_red_alph_N" amino acids 3 to 40 (38 residues), 72.3 bits, see alignment (E = 4.9e-24) PF00384: Molybdopterin" amino acids 108 to 852 (745 residues), 229.4 bits, see alignment E=1e-71 PF01568: Molydop_binding" amino acids 1108 to 1223 (116 residues), 77.1 bits, see alignment E=1.6e-25

Best Hits

KEGG orthology group: K00370, nitrate reductase 1, alpha subunit [EC: 1.7.99.4] (inferred from 84% identity to ajs:Ajs_0436)

MetaCyc: 69% identical to respiratory nitrate reductase alpha subunit (Stutzerimonas stutzeri)
RXN-11236 [EC: 1.7.5.1]

Predicted SEED Role

"Respiratory nitrate reductase alpha chain (EC 1.7.99.4)" in subsystem Nitrate and nitrite ammonification (EC 1.7.99.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.7.99.4

Use Curated BLAST to search for 1.7.5.1 or 1.7.99.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0C4Y8B8 at UniProt or InterPro

Protein Sequence (1267 amino acids)

>RR42_RS08915 nitrate reductase (Cupriavidus basilensis FW507-4G11)
MSHFLDRLTYFSQPRESFSDGHGQTTGEDRTWEDAYRNRWQHDKIVRSTHGTNCTGSCSW
KIYVKGGIVTWETQQTDYPRTRADLPNHEPRGCARGASYSWYLYSANRVKYPMVRGRLLK
HWRSARAVAKSPVDAWAAVVEDDAARREYQQQRGLGGFVRSSWDEVNELIAAANVYTIKK
HGPDRVIGFSPIPAMSMVSYAAGSRYLSLIGGVCMSFYDWYCDLPPASPQTWGEQTDVPE
SADWYNSTFLIAWGSNVPQTRTPDAHFFTEVRYKGAKVVAVTPDYAEVSKLADLWLHPKQ
GTDAALAMAMGHVILKEFYFERRSAYFDDYARRYTDLPLLVALKEHTLPDGSKTLVPDRY
VRASDFPDALGQANHPEWKTVGFDEQGKVALPHGSIGFRWGPDGRPDAGKWNLESKEARQ
GEAVKLKLSVVEDGAQPHDIVDVGFPYFGGIDTPHFTSSKTAGDVIVRRVPAARLALQEA
GGQRETLVATVFDLLAANYGVARGLPGEQQGGAATGGYDDEVPYTPAWQERITGVARQQV
IAVARQFADNADKTRGKSMVIIGAAMNHWYHCDMNYRGIINMLMLCGCIGQSGGGWAHYV
GQEKLRPQTGWTALAFALDWIRPPRQMNSTSFFYAHTDQWRYEKLGVDEILSPLADPARY
SGSMIDYNVRAERMGWLPSAPQLQTNPLQVQRDAQARGMDTRDYVAQSLKDGSLKLSCED
PDHPANWPRNMFVWRSNLLGSSGKGHEYFLKHLLGTSHGVQGKDLGPDHAKPEEVRWHEQ
APEGKLDLLVTLDFRMSTTCMYSDIVLPTATWYEKNDLNTSDMHPFIHPLSTAVDPAWQA
RSDWEIYKGFAKTFSKVCAGHLGVEKEVVLTPLMHDTPAELAQPFGVRDWKKGECDLVPG
QTAPQINVVERDYPNTYQRFTALGPLMTRIGNGGKGIGWDTQSEVHALGELNGTVSEAGV
SQGLPRIDTDIDAAEVVMMLAPETNGHVAVKAWQALSRITGREHAHLALHREDEKIRFRD
IQAQPRKIISSPTWSGLESEKVSYNAGYTNVHELIPWRTLSGRQQFYQDHPWMRDFGEGF
VSYRPPINLKNVDEMLGKKPNGHPEIALNFITPHQKWGIHSTYTDNLIMLTLNRGGPVIW
LSEDDAGRAGIVDNDWVELFNANGAIAARAVVSQRIKPGMVMMYHAQEKIINTPGSEITG
LRGGIHNSVTRVVLKPTHMIGGYAQYSYGFNYYGTIGTNRDEFVVVRKMNRVDWLDEPAN
ADAPGAA