Protein Info for RR42_RS05760 in Cupriavidus basilensis FW507-4G11

Annotation: 3-hydroxyacyl-CoA dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

KEGG orthology group: K00074, 3-hydroxybutyryl-CoA dehydrogenase [EC: 1.1.1.157] (inferred from 88% identity to cti:RALTA_A1087)

MetaCyc: 88% identical to 3-hydroxyacyl-CoA dehydrogenase (Cupriavidus necator H16)
3-hydroxyacyl-CoA dehydrogenase. [EC: 1.1.1.35]

Predicted SEED Role

"3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35) / Enoyl CoA hydratase (EC 4.2.1.17)" in subsystem Acetyl-CoA fermentation to Butyrate or Isoleucine degradation or Polyhydroxybutyrate metabolism or Valine degradation or n-Phenylalkanoic acid degradation (EC 1.1.1.35, EC 4.2.1.17)

MetaCyc Pathways

oleate β-oxidation (29/35 steps found)
adipate biosynthesis (5/5 steps found)
adipate degradation (5/5 steps found)
superpathway of phenylethylamine degradation (9/11 steps found)
benzoyl-CoA degradation I (aerobic) (6/7 steps found)
superpathway of glyoxylate cycle and fatty acid degradation (11/14 steps found)
benzoyl-CoA biosynthesis (3/3 steps found)
fatty acid salvage (5/6 steps found)
phenylacetate degradation I (aerobic) (7/9 steps found)
4-hydroxybenzoate biosynthesis III (plants) (4/5 steps found)
L-valine degradation I (6/8 steps found)
fatty acid β-oxidation I (generic) (5/7 steps found)
2-methyl-branched fatty acid β-oxidation (10/14 steps found)
L-glutamate degradation V (via hydroxyglutarate) (7/10 steps found)
L-isoleucine degradation I (4/6 steps found)
propanoate fermentation to 2-methylbutanoate (4/6 steps found)
pyruvate fermentation to butanol II (engineered) (4/6 steps found)
superpathway of Clostridium acetobutylicum acidogenic fermentation (6/9 steps found)
valproate β-oxidation (6/9 steps found)
(R)- and (S)-3-hydroxybutanoate biosynthesis (engineered) (3/5 steps found)
fatty acid β-oxidation II (plant peroxisome) (3/5 steps found)
fatty acid β-oxidation IV (unsaturated, even number) (3/5 steps found)
glutaryl-CoA degradation (3/5 steps found)
L-tryptophan degradation III (eukaryotic) (10/15 steps found)
pyruvate fermentation to hexanol (engineered) (7/11 steps found)
pyruvate fermentation to butanoate (4/7 steps found)
3-phenylpropanoate degradation (6/10 steps found)
6-gingerol analog biosynthesis (engineered) (3/6 steps found)
methyl ketone biosynthesis (engineered) (3/6 steps found)
1-butanol autotrophic biosynthesis (engineered) (18/27 steps found)
photosynthetic 3-hydroxybutanoate biosynthesis (engineered) (17/26 steps found)
(8E,10E)-dodeca-8,10-dienol biosynthesis (6/11 steps found)
androstenedione degradation I (aerobic) (16/25 steps found)
fatty acid β-oxidation VI (mammalian peroxisome) (3/7 steps found)
superpathway of Clostridium acetobutylicum acidogenic and solventogenic fermentation (10/17 steps found)
superpathway of Clostridium acetobutylicum solventogenic fermentation (7/13 steps found)
benzoate biosynthesis I (CoA-dependent, β-oxidative) (4/9 steps found)
glycerol degradation to butanol (9/16 steps found)
benzoate biosynthesis III (CoA-dependent, non-β-oxidative) (1/5 steps found)
pyruvate fermentation to butanol I (3/8 steps found)
3-hydroxypropanoate/4-hydroxybutanate cycle (10/18 steps found)
superpathway of testosterone and androsterone degradation (17/28 steps found)
2-methylpropene degradation (2/8 steps found)
methyl tert-butyl ether degradation (3/10 steps found)
L-glutamate degradation VII (to butanoate) (4/12 steps found)
benzoate fermentation (to acetate and cyclohexane carboxylate) (7/17 steps found)
crotonate fermentation (to acetate and cyclohexane carboxylate) (6/16 steps found)
(4Z,7Z,10Z,13Z,16Z)-docosapentaenoate biosynthesis (6-desaturase) (2/13 steps found)
toluene degradation VI (anaerobic) (5/18 steps found)
docosahexaenoate biosynthesis III (6-desaturase, mammals) (2/14 steps found)
cholesterol degradation to androstenedione I (cholesterol oxidase) (4/17 steps found)
androstenedione degradation II (anaerobic) (10/27 steps found)
superpathway of cholesterol degradation I (cholesterol oxidase) (20/42 steps found)
platensimycin biosynthesis (7/26 steps found)
Spodoptera littoralis pheromone biosynthesis (4/22 steps found)
cholesterol degradation to androstenedione II (cholesterol dehydrogenase) (4/22 steps found)
superpathway of cholesterol degradation II (cholesterol dehydrogenase) (20/47 steps found)
superpathway of cholesterol degradation III (oxidase) (12/49 steps found)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.157, 1.1.1.35, 4.2.1.17

Use Curated BLAST to search for 1.1.1.157 or 1.1.1.35 or 4.2.1.17

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0C4Y0V3 at UniProt or InterPro

Protein Sequence (507 amino acids)

>RR42_RS05760 3-hydroxyacyl-CoA dehydrogenase (Cupriavidus basilensis FW507-4G11)
MSTFTIDSLGIVGTGAMGRGIAQIAAQAGLTVSLYDTNPQAVAAARQYLQDTFAKLAEKG
KMSAEDGAAALARVLPCNVLEDLAGCDMVLEAIVEKLEVKRDLVARLEAVLRADAVIASN
TSSLSITAIAVGAKQPGRIAGYHFFNPVPLMKVVEVIDGLSGDPAVGDALMALSRRLGHT
PVRCKDMPGFIVNHAGRGMNIEGLKVAQEGVADFADIDAIMREQAGFRMGPFELMDLTGL
DVSHPVMESIYNQFYQEPRYRPSPITAIRSVGGLIGRKAGAGFYRYADGQKQLPATAALP
AARPASVWVSRADERGHDMAVKLILALGMTPEAGNHPSGDALIVVTPLGLDATTCALQEG
LDPARVIAIDTLLPFEATKRRTLMTTPATSAAMREAAHGLFGSDGVPVTVIRDSAGFVAQ
RIISCIVNIASDIAQQRIATPVDIDLAVNLGLGYPKGPLALGDVIGPHLVLTVLRNLERL
TGDMRYRPSPWLWRRAGLKLSLLAEEQ