Protein Info for RR42_RS04880 in Cupriavidus basilensis FW507-4G11

Annotation: coproporphyrinogen III oxidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 416 TIGR00539: putative oxygen-independent coproporphyrinogen III oxidase" amino acids 38 to 391 (354 residues), 360.2 bits, see alignment E=6.3e-112 PF04055: Radical_SAM" amino acids 41 to 216 (176 residues), 81.8 bits, see alignment E=6.8e-27 PF06969: HemN_C" amino acids 343 to 405 (63 residues), 41.9 bits, see alignment E=8.6e-15

Best Hits

Swiss-Prot: 53% identical to HEMW_ECOLI: Heme chaperone HemW (hemW) from Escherichia coli (strain K12)

KEGG orthology group: K02495, oxygen-independent coproporphyrinogen III oxidase [EC: 1.3.99.22] (inferred from 86% identity to cti:RALTA_A0944)

Predicted SEED Role

"Radical SAM family enzyme, similar to coproporphyrinogen III oxidase, oxygen-independent, clustered with nucleoside-triphosphatase RdgB" in subsystem Heat shock dnaK gene cluster extended or Heme and Siroheme Biosynthesis or Queuosine-Archaeosine Biosynthesis

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.3.99.22

Use Curated BLAST to search for 1.3.99.22

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0C4Y874 at UniProt or InterPro

Protein Sequence (416 amino acids)

>RR42_RS04880 coproporphyrinogen III oxidase (Cupriavidus basilensis FW507-4G11)
MIPIVPLASTGAGARAPADTKSLWLQPGQLALPGSPPLSLYVHIPWCVRKCPYCDFNSHA
VPGATDSHDIPEDAYLDALRADLEQSLPLVWGRQVHTVFIGGGTPSLLSAAGMDRLLSDI
RALLPLDADAEITMEANPGTFEAERFASYRASGINRLSIGIQSFNDRHLAALGRIHGGAE
ARAAIGIALDNFDNVNLDLMYALPGQTMAECADDLETALSFNTTHLSLYHLTLEPNTLFA
KFPPALPDDDSAYEMQDLIEARTAQAGYVHYETSAYARPHRQARHNLNYWRFGDYLGIGA
GAHGKLSFPHRVLRQMRHKHPATYMAQAMAGNAVQEQHDVGAADLPFEFMLNALRLTDGV
PASTFFDHTGLPLHTISRDLAAAEKKGLLEADLSTIKPTELGRRFLNDLQEMFLKG