Protein Info for RR42_RS02720 in Cupriavidus basilensis FW507-4G11

Annotation: phosphoribosylaminoimidazolecarboxamide formyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00355: phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase
  • amino acids 3 to 524 (522 residues), 746.3 bits, E=9.8e-229
• PF02142: MGS
  • amino acids 14 to 129 (116 residues), 96.9 bits, E=6.3e-32
• PF01808: AICARFT_IMPCHas
  • amino acids 134 to 454 (321 residues), 416.8 bits, E=6.4e-129

Best Hits

Swiss-Prot: 94% identical to PUR9_CUPNH: Bifunctional purine biosynthesis protein PurH (purH) from Cupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337)

KEGG orthology group: K00602, phosphoribosylaminoimidazolecarboxamide formyltransferase / IMP cyclohydrolase [EC: 2.1.2.3 3.5.4.10] (inferred from 94% identity to cti:RALTA_A0457)

Predicted SEED Role

"IMP cyclohydrolase (EC 3.5.4.10) / Phosphoribosylaminoimidazolecarboxamide formyltransferase (EC 2.1.2.3)" in subsystem De Novo Purine Biosynthesis (EC 2.1.2.3, EC 3.5.4.10)

MetaCyc Pathways

• superpathway of histidine, purine, and pyrimidine biosynthesis (44/46 steps found)
• superpathway of purine nucleotides de novo biosynthesis I (21/21 steps found)
• superpathway of purine nucleotides de novo biosynthesis II (24/26 steps found)
• inosine-5'-phosphate biosynthesis I (6/6 steps found)
• inosine-5'-phosphate biosynthesis II (5/5 steps found)
• inosine-5'-phosphate biosynthesis III (5/6 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Biosynthesis of plant hormones
• One carbon pool by folate
• Purine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.1.2.3 or 3.5.4.10

Sequence Analysis Tools

See A0A0C4XZJ5 at UniProt or InterPro

Protein Sequence (524 amino acids)

>RR42_RS02720 phosphoribosylaminoimidazolecarboxamide formyltransferase (Cupriavidus basilensis FW507-4G11)
MIKQALLSVSDKTGIVEFARELNSLGVALLSTGGTAKLLADAGLPVTEVADYTGFPEMLD
GRVKTLHPKVHGGILARRDLPEHMAALAEHNIPTIDLLVVNLYPFQQTVAKDDCSLPDAI
ENIDIGGPTMLRSAAKNHRDVTVIVDPADYAPVLEEMRANANSVGYDTNFRLATKVFAHT
AQYDGAITNYLTSLGADKAHQTRSAYPQTLNLAFEKVQEMRYGENPHQSAAFYRDLKASD
GALANYTQLQGKELSYNNIADADAAWECVKTFGAATGAACVIIKHANPCGVAVGANALEA
YEKALKTDSTSAFGGIIAFNVELDEAAAQAVAKQFVEVLIAPSFSAGARAVFAAKQNVRL
LEIPLGNGLNPYDFKRVGGGLLVQGPDARNVQRDELRVVTKRQPTPKEMDDLMFAWRVAK
FVKSNAIVFCGGGMTLGVGAGQMSRVDSARIASIKAQNAGLTLAGSAVASDAFFPFRDGL
DVVVDAGATCVIQPGGSMRDDEVIAAADERNIAMVLTGVRHFRH