Protein Info for RR42_RS02325 in Cupriavidus basilensis FW507-4G11

Annotation: glucoamylase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 612 PF19291: TREH_N" amino acids 19 to 167 (149 residues), 56.9 bits, see alignment E=1.9e-19 PF00723: Glyco_hydro_15" amino acids 238 to 599 (362 residues), 153.6 bits, see alignment E=8.5e-49

Best Hits

KEGG orthology group: None (inferred from 85% identity to reh:H16_A0429)

Predicted SEED Role

"Glucoamylase (EC 3.2.1.3)" in subsystem Maltose and Maltodextrin Utilization or Trehalose Biosynthesis (EC 3.2.1.3)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.2.1.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0C4YBC2 at UniProt or InterPro

Protein Sequence (612 amino acids)

>RR42_RS02325 glucoamylase (Cupriavidus basilensis FW507-4G11)
MSNPSTEEFPARAGDDGRYAAPSLSLGMIGNCAISALVDGRGRIVWCCLPRFDGDPVFNA
LLDPGENAGHFAIEIEDFHEAHQWYEPNTAVLRTRLTDQHGNSLEITDFAPRFFRIGRYF
RPTSLIRRIRPVQGAPRVRVTLAPRFDWGRVKPEITRGSSHVRYIGDGSTLRMTTDAPLT
YVLSQTPFLLTRDLNFILGPDETLQGGVEEIARDFEQETTAYWKMWSRRLAVPREWQEAV
IRAAITLKLSLYEETGAIVAAMTTSIPEAPGSGRNWDYRFCWLRDAFFVIRALNSLSEVG
TMEDYLRWLSNVVMQSRNGHIQPLYGIGLELELPESMLDHLSGYRGMGPVRVGNQAQEHF
QHDVYGNVVLGAAQAFHDHRLLHRGGAAEYHVLETVGEQAVRVFGTPDAGMWELRTRARV
HTSSALMSWAACDRLAKIGDALQLPDRAAYWRQHAEGMKVRIVRESWSEERQAFAESFGG
RELDASVLLMAEVGFIEPRDERFMSTLKAMEASLCDGPYMRRYEAPDDFGKPETAFNICT
FWRIDALARVGRREEAREIFEAMLAARNPLGLLSEDTHPVTGEMWGNFPQTYSMVGLING
AMRLSAPWDSVI