Protein Info for RR42_RS01810 in Cupriavidus basilensis FW507-4G11

Annotation: tRNA 2-selenouridine synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 374 TIGR03167: tRNA 2-selenouridine synthase" amino acids 16 to 355 (340 residues), 347.8 bits, see alignment E=3.2e-108

Best Hits

Swiss-Prot: 70% identical to SELU_CUPNH: tRNA 2-selenouridine/geranyl-2-thiouridine synthase (selU) from Cupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337)

KEGG orthology group: K06917, tRNA 2-selenouridine synthase [EC: 2.9.1.-] (inferred from 70% identity to reh:H16_A0329)

MetaCyc: 45% identical to tRNA 2-selenouridine synthase (Escherichia coli K-12 substr. MG1655)
RXN0-2281 [EC: 2.9.1.3]

Predicted SEED Role

"Selenophosphate-dependent tRNA 2-selenouridine synthase" in subsystem Selenocysteine metabolism

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.9.1.- or 2.9.1.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0C4Y4M6 at UniProt or InterPro

Protein Sequence (374 amino acids)

>RR42_RS01810 tRNA 2-selenouridine synthase (Cupriavidus basilensis FW507-4G11)
MREDTADFRQLFLSGAPLLDARAPVEFARGAFPGAVNLPLMDDAERHQVGLCYEEQGPQA
ALALGHQLVNGEIRATRTAGWAAFARAHPDGYLYCFRGGLRSQIVQQWLREDAGIAYPRV
TGGYKAMRSFLIETLDAAAAECSLLVLGGMTGSGKTELIADLVTGQGGSRDAAIDLEGQA
RHRGSSFGRRTVAQPTQIDFENGLAISLLRHRAAGLPALVVEDEGRFIGARNLPQAFYQA
IQVAPLVWLEESFEARVHRVLGDYVLGLSAEFMAALGEQAGFAAYAARLRDAMAAIAKRL
GGTRYERLSDLLDQALARQEETGETDLHLGWIALLLQQYYDPMYAYQREQREARIVFRGE
RAAVLAFLRERTRA