Protein Info for RR42_RS01420 in Cupriavidus basilensis FW507-4G11

Annotation: 2', 3'-cyclic nucleotide 2'-phosphodiesterase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 411 PF01743: PolyA_pol" amino acids 3 to 127 (125 residues), 56.8 bits, see alignment E=4.5e-19 PF12627: PolyA_pol_RNAbd" amino acids 153 to 215 (63 residues), 42.5 bits, see alignment E=7e-15 PF01966: HD" amino acids 234 to 325 (92 residues), 38.6 bits, see alignment E=1.8e-13

Best Hits

Swiss-Prot: 84% identical to CCA_CUPNH: Multifunctional CCA protein (cca) from Cupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337)

KEGG orthology group: K00974, tRNA nucleotidyltransferase (CCA-adding enzyme) [EC: 2.7.7.72 3.1.3.- 3.1.4.-] (inferred from 84% identity to reh:H16_A0253)

MetaCyc: 56% identical to fused CCA tRNA nucleotidyltransferase/phosphohydrolase (Escherichia coli K-12 substr. MG1655)
TRNA-CYTIDYLYLTRANSFERASE-RXN [EC: 2.7.7.72]

Predicted SEED Role

"tRNA nucleotidyltransferase (EC 2.7.7.21) (EC 2.7.7.25)" in subsystem Polyadenylation bacterial or tRNA nucleotidyltransferase (EC 2.7.7.21, EC 2.7.7.25)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.1.3.-, 3.1.4.-

Use Curated BLAST to search for 2.7.7.21 or 2.7.7.25 or 2.7.7.72 or 3.1.3.- or 3.1.4.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0C4YAY6 at UniProt or InterPro

Protein Sequence (411 amino acids)

>RR42_RS01420 2', 3'-cyclic nucleotide 2'-phosphodiesterase (Cupriavidus basilensis FW507-4G11)
MQVYAVGGAIRDALLGLSSQDRDYVVVGATPAEMEAQGYKPVGKDFPVFLHPRTREEYAL
ARTERKTAAGYKGFSFYCAPDVTLEDDLVRRDLTINAMARAVDDDGALAGPVIDPYGGQR
DLRARLFRHVSDAFAEDPVRILRVARFAARFHDFAVAPETRVLMRRMVEAGEVDALVAER
VWQELARGLMEARPSRMFEVLRECGALARLLPELDRLWGVPQRADYHPEVDTGVHVMMVV
DTAAAMGASLPVRFAALMHDLGKGTTPEDVLPRHIGHEQRSVALLEAVCKRLRVPTDCRD
LAVVVAREHGNIHRSEAFGAAALTRLLERCDALRKPARFAQALQACEADARGRLGFEDRE
YPQTARLMMALEAAASVDAGAVARECAGDPGLIKDRVHVARVAAVAVKVGG