Protein Info for RR42_RS00775 in Cupriavidus basilensis FW507-4G11

Annotation: methionine synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02082: methionine synthase
  • amino acids 9 to 885 (877 residues), 1204.3 bits, E=0
• PF00809: Pterin_bind
  • amino acids 31 to 269 (239 residues), 212.2 bits, E=2e-66
• PF02607: B12-binding_2
  • amino acids 333 to 409 (77 residues), 78.6 bits, E=6.4e-26
• PF02310: B12-binding
  • amino acids 431 to 524 (94 residues), 61.4 bits, E=1.6e-20
• PF02965: Met_synt_B12
  • amino acids 625 to 901 (277 residues), 400.3 bits, E=9.2e-124

Best Hits

KEGG orthology group: K00548, 5-methyltetrahydrofolate--homocysteine methyltransferase [EC: 2.1.1.13] (inferred from 84% identity to bcm:Bcenmc03_2932)

Predicted SEED Role

"5-methyltetrahydrofolate--homocysteine methyltransferase (EC 2.1.1.13)" in subsystem Methionine Biosynthesis (EC 2.1.1.13)

MetaCyc Pathways

• aspartate superpathway (23/25 steps found)
• superpathway of L-methionine biosynthesis (by sulfhydrylation) (12/12 steps found)
• superpathway of L-lysine, L-threonine and L-methionine biosynthesis I (16/18 steps found)
• folate transformations III (E. coli) (9/9 steps found)
• folate transformations II (plants) (10/11 steps found)
• L-methionine biosynthesis III (4/4 steps found)
• superpathway of S-adenosyl-L-methionine biosynthesis (7/9 steps found)
• superpathway of L-methionine biosynthesis (transsulfuration) (7/9 steps found)
• superpathway of L-homoserine and L-methionine biosynthesis (6/8 steps found)
• folate transformations I (9/13 steps found)
• L-methionine biosynthesis I (3/5 steps found)
• L-methionine salvage from L-homocysteine (1/3 steps found)
• superpathway of L-methionine salvage and degradation (7/16 steps found)

KEGG Metabolic Maps

• Methionine metabolism
• One carbon pool by folate

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.13

Use Curated BLAST to search for 2.1.1.13

Sequence Analysis Tools

See A0A0C4Y440 at UniProt or InterPro

Protein Sequence (915 amino acids)

>RR42_RS00775 methionine synthase (Cupriavidus basilensis FW507-4G11)
MTNDTLPPRPMRLSGLEPFTIDEDTLFVNVGERTNVTGSKAFARMILNGQFDDALVVARQ
QVENGAQIIDINMDEAMLDSKAAMVRFLNLIASEPDIARVPIMLDSSKWEVIEAGLKCVQ
GKPVVNSISLKEGEEQFRHHAELIRRYGAASVVMAFDEQGQADTFARKTEICKRSYDILV
NEVGFPPEDIIFDPNIFAVATGIEEHNNYAVDFIEATAWIKQNLPYAKVSGGVSNVSFSF
RGNDAVREAIHTVFLYHAIKAGMDMGIVNAGQLGVYDQLDAELRERVEDVVLNRREDSTD
RLLEIADRYKGGGAKKEENLLWRGTPENPVPVADRLSHALVHGLTTFIVEDTEEVRQQVE
ARGGRTIEVIEGPLMDGMNIVGDLFGAGKMFLPQVVKSARVMKQAVAHLLPYIEEEKRLL
AEAGGDVKARGKIVIATVKGDVHDIGKNIVSVVLQCNNFEVVNMGVMVPCNEILARAKVE
GADIVGLSGLITPSLEEMAYVASEMQRDDYFRIKKIPLLIGGATTSRVHTAVKIAPHYEG
PVVYVPDASRSVSVASSLLSDDGAARYLDELKTDYERIRHQHANKKATPMVSLAKARANK
TPVDWSAYVPPKPKFIGRRIFRNYDLTELANYIDWAPFFQTWDLAGKFPDILNDEIVGES
ARRVFSDGKAMLSRLIQGRWLTANGVLALLPANAVNDDDIEIYTDETRSQVALTWHNLRQ
QSERPVIDGVMRPNRCLADFVAPKDSGIADYIGVFAVTAGIGVDKKEAQFEADHDDYSAI
MLKSLADRLAEAFAECLHERVRRDLWGYDAGEVLTNEQLIAETYRGIRPAPGYPACPEHT
VKAPMFEFLNAAEIGMGITDSLAMTPAASVSGFYLAHPESTYFSVGKIGEDQLDDMVARR
GEERSVLERALAPNL