Protein Info for RR42_RS00590 in Cupriavidus basilensis FW507-4G11
Annotation: glutamyl-tRNA amidotransferase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 91% identical to GATA_CUPTR: Glutamyl-tRNA(Gln) amidotransferase subunit A (gatA) from Cupriavidus taiwanensis (strain DSM 17343 / BCRC 17206 / CIP 107171 / LMG 19424 / R1)
KEGG orthology group: K02433, aspartyl-tRNA(Asn)/glutamyl-tRNA (Gln) amidotransferase subunit A [EC: 6.3.5.6 6.3.5.7] (inferred from 91% identity to cti:RALTA_A0051)Predicted SEED Role
"Aspartyl-tRNA(Asn) amidotransferase subunit A (EC 6.3.5.6) @ Glutamyl-tRNA(Gln) amidotransferase subunit A (EC 6.3.5.7)" (EC 6.3.5.6, EC 6.3.5.7)
MetaCyc Pathways
- L-glutamate and L-glutamine biosynthesis (7/7 steps found)
- L-asparagine biosynthesis III (tRNA-dependent) (4/4 steps found)
- ammonia assimilation cycle III (3/3 steps found)
- L-glutamate biosynthesis I (2/2 steps found)
- L-glutamine degradation I (1/1 steps found)
- L-citrulline biosynthesis (6/8 steps found)
- glutaminyl-tRNAgln biosynthesis via transamidation (3/4 steps found)
- superpathway of L-citrulline metabolism (8/12 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 6.3.5.6, 6.3.5.7
Use Curated BLAST to search for 6.3.5.6 or 6.3.5.7
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A0C4Y406 at UniProt or InterPro
Protein Sequence (501 amino acids)
>RR42_RS00590 glutamyl-tRNA amidotransferase (Cupriavidus basilensis FW507-4G11) MPFSATSVTSLRELSDALAARSVSAEELARDYLARIEQAGALNAFVDVNPELTLAQARAA DARRARGEAGPLTGVPIAHKDVFVTRGWRATAGSRMLANYESPFDAAVVERLAAAGMVTL GKTNMDEFAMGSSNENSHFGPVRNPWDASRVPGGSSGGSAAAVAAGLAPAATGTDTGGSI RQPASFSGITGIKPTYGRVSRYGMIAFASSLDQGGPMAHTAEDCAMLLGGMAGFDERDST SVAPALGGVDEDYTRLLGQARAGASAGKPLSGLRIGLPKEYFGKGLAADVEQAVRAALAE YEKLGATLVEVSLPKTELSIPVYYVIAPAEASSNLSRFDGVRFGHRAAEYRDLMDMYKKT RAEGFGPEVKRRIMVGAYVLSHGYYDAYYLQAQKIRRIIADDFQRAFTQCDVIMGPVAPT VAWKLGEKSADPVQMYLADIFTLSTSLAGLPGMSVPCGLGEGGMPVGLQLIGNYFGEAEL LQTAHAFQQATDWHLRRPAQA