Protein Info for RALBFv3_RS23235 in Ralstonia solanacearum IBSBF1503

Annotation: MFS transporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 448 transmembrane" amino acids 40 to 60 (21 residues), see Phobius details amino acids 73 to 95 (23 residues), see Phobius details amino acids 106 to 124 (19 residues), see Phobius details amino acids 130 to 151 (22 residues), see Phobius details amino acids 175 to 195 (21 residues), see Phobius details amino acids 207 to 226 (20 residues), see Phobius details amino acids 258 to 277 (20 residues), see Phobius details amino acids 296 to 314 (19 residues), see Phobius details amino acids 325 to 345 (21 residues), see Phobius details amino acids 351 to 374 (24 residues), see Phobius details amino acids 386 to 407 (22 residues), see Phobius details amino acids 419 to 437 (19 residues), see Phobius details PF00083: Sugar_tr" amino acids 39 to 239 (201 residues), 102.5 bits, see alignment E=2.7e-33 amino acids 239 to 441 (203 residues), 50.2 bits, see alignment E=2e-17 PF07690: MFS_1" amino acids 41 to 279 (239 residues), 58.9 bits, see alignment E=4.3e-20 amino acids 271 to 444 (174 residues), 48.8 bits, see alignment E=5.1e-17

Best Hits

Swiss-Prot: 54% identical to KGTP_ECOLI: Alpha-ketoglutarate permease (kgtP) from Escherichia coli (strain K12)

KEGG orthology group: K03761, MFS transporter, MHS family, alpha-ketoglutarate permease (inferred from 96% identity to rsl:RPSI07_mp0008)

MetaCyc: 54% identical to alpha-ketoglutarate:H+ symporter (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-23

Predicted SEED Role

"Alpha-ketoglutarate permease"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (448 amino acids)

>RALBFv3_RS23235 MFS transporter (Ralstonia solanacearum IBSBF1503)
MHQPQATVVAGAHVPAHETVHERSADDVSKRLRSIFSGSVGNLVEWYDWYVYSAFALYFA
KSFFPKGDQTAQLLNTAAVFAVGFLARPVGGWLMGIYADRHGRKRALLVSVVLMCMGSLI
IALTPSYATIGVAAPTLLVLARLLQGLSVGGEYGTSATYLSEVATRESRGFYSSFQYVTL
IAGQLVALALLIVLQQFVLSPAQLDAWGWRIPFFIGALCALIAMRLRSSMEETQSFTSTT
MDKKKRGSMRELARHPRAVATVVGLTLGGTVAFYTYSIYMQKFLANTVGLSKDQATLVSG
ATLLLFALLQPIVGGLSDRVGRRPILIAFGVLGTLFTVPIMTGISQTSNPWTAFFLIMAA
LVIVSGYTSINAVVKAELFPTEVRALGVGLPYALTVSIFGGTAEYIALWLKQAGHEPWFY
WYVTGAIFLSLMVYVFMRDTRAHSRIEH