Protein Info for RALBFv3_RS22995 in Ralstonia solanacearum IBSBF1503

Annotation: MFS transporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 437 transmembrane" amino acids 23 to 50 (28 residues), see Phobius details amino acids 63 to 84 (22 residues), see Phobius details amino acids 95 to 114 (20 residues), see Phobius details amino acids 120 to 141 (22 residues), see Phobius details amino acids 161 to 184 (24 residues), see Phobius details amino acids 196 to 215 (20 residues), see Phobius details amino acids 245 to 264 (20 residues), see Phobius details amino acids 283 to 303 (21 residues), see Phobius details amino acids 313 to 332 (20 residues), see Phobius details amino acids 338 to 361 (24 residues), see Phobius details amino acids 373 to 394 (22 residues), see Phobius details amino acids 406 to 424 (19 residues), see Phobius details PF00083: Sugar_tr" amino acids 26 to 222 (197 residues), 79.6 bits, see alignment E=2.3e-26 amino acids 231 to 423 (193 residues), 37.2 bits, see alignment E=1.8e-13 TIGR00883: MFS transporter, metabolite:H+ symporter (MHS) family protein" amino acids 31 to 416 (386 residues), 447.5 bits, see alignment E=2.2e-138 PF07690: MFS_1" amino acids 69 to 383 (315 residues), 83.9 bits, see alignment E=1.1e-27 amino acids 274 to 429 (156 residues), 42.2 bits, see alignment E=5.3e-15

Best Hits

Swiss-Prot: 70% identical to KGTP_SHIFL: Alpha-ketoglutarate permease (kgtP) from Shigella flexneri

KEGG orthology group: K03761, MFS transporter, MHS family, alpha-ketoglutarate permease (inferred from 96% identity to rso:RSp0075)

MetaCyc: 70% identical to alpha-ketoglutarate:H+ symporter (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-23

Predicted SEED Role

"dicarboxylic acid transporter PcaT" in subsystem Protocatechuate branch of beta-ketoadipate pathway

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (437 amino acids)

>RALBFv3_RS22995 MFS transporter (Ralstonia solanacearum IBSBF1503)
MSTPAAQASQAAMAPHDTRRRIFAIVGASSGNLVEWFDFYVYAFCSLYFAPAFFPSGNTT
TQLMNTAGVFAAGFLMRPIGGWLFGRIADQHGRRIAMMISVLMMCGGSLVIAVLPTYAQI
GALAPLLLLLARLFQGLSVGGEYGTSATYMSEVALKGRRGFFASFQYVTLIGGQLCALLV
LVTLQQILSTAELKAWGWRIPFVVGALSALISLYLRRSLDETQSTASREAKHAGTIRGAW
QHKGAFLRVIGFTAGGSLIFYTFTTYMQKYLVNTAGMNTKTASNVMTAALFVYMVLQPVF
GALSDRIGRRMSMILFGVGAVLFTVPLMRALGSVTSPYAAFGLIIVALAIISFYTSISGL
IKAEMFPPEVRAMGVGLSYAIANAVFGGSAEYVALWFKSAGSESTFYWYVTILCAVSLIV
SYTMPDPSKEGYLRHEP