Protein Info for RALBFv3_RS22295 in Ralstonia solanacearum IBSBF1503

Annotation: DUF3416 domain-containing protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1100 1232 PF11896: GlgE_dom_N_S" amino acids 558 to 743 (186 residues), 188.2 bits, see alignment E=2.6e-59 PF00128: Alpha-amylase" amino acids 756 to 881 (126 residues), 24.4 bits, see alignment E=2.6e-09 PF21702: GLGE_C" amino acids 1153 to 1214 (62 residues), 42 bits, see alignment 1.3e-14

Best Hits

KEGG orthology group: None (inferred from 90% identity to rsl:RPSI07_mp0203)

Predicted SEED Role

"Alpha-amylase (EC 3.2.1.1)" in subsystem Maltose and Maltodextrin Utilization or Trehalose Biosynthesis (EC 3.2.1.1)

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.2.1.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (1232 amino acids)

>RALBFv3_RS22295 DUF3416 domain-containing protein (Ralstonia solanacearum IBSBF1503)
MTAPKTFATSAPRTPPSATSATSATTRTRRAAGDALRPVGKPASAEGQTPTMQAPSRGHP
AESRWTQPDARRRATVFAPAICYLHPLQHGPLDQWGSLLDRIASLGFDHVLIPPPFAVGA
GGNVFVTRDHARLHAALGGGPADAALRRLADDCRQRGLGLLMDLVIDRVATDAPLRADHP
DWFASRTEHGDALDPRLPPSHLEVASFRHDDARCADAIGAWWAHQLRAWTALGIDGFRCE
APARLPPARWRTLIAATREATAARPVRFLAWTPGLDGEAVDALAQAGFDATFSSLRWWDF
RAGWMVEEHARLAAIAPPIATVEVPFGTRYGQAFGDAMTRERAYRRMLDVADVLGAGWLM
PFGFERGAMLPMLSERGDPTDQQWIHAHAAFDLSDAVRDVNATLRAAHADGTVAPRAELR
MLTGPDARMSALLRADGPDLRADEAATLLAINPDLHVGVSAHADRFLPGVAGGFTRGVAL
DLLTEPTGSHDDALRTEAHPLAEIDLPPGGVQVWRVFRSRPVRRRPGVAPVIAPGVQQAE
PARGDADNARANSAVAAPRIAIEHVTPAVDDGRFAVKRLVGDDILVEADVLMDGHDELAV
ELLWRADDDAWQRVPMVPLGNDRWRGAFRPERLGRHRYAVAAWRGAFGTFRAELEKKHTA
GVDVSVELEEGAQLVAHAAGHAPKNAPLGALHQLADRLQTTAQDAAHRLKLLLDPATAHV
MAQASHFRPFHVQSAPYPLEVERSGAAFANWYELFPRSASGDEGRHGTFRDVVGRLGTIR
AMGFDVLYFPPIHPIGRTNRKGRNNSLRAGPGEPGSPYAIGAAEGGHDAVHPQLGTLEDF
RRLRDAAREQGLELALDFAIQCSPDHPWLREHPGWFAWRSDGSVRYAENPPKKYEDIVNV
DFYAADAKPALWRALRDVVMFWADEGVRLFRVDNPHTKPLPFWEWMIGEVRARHPDAIFL
SEAFTRPKGMYRLAKVGFSQSYTYFTWRHTRQEFTDYLTELTRTPVREYFRPHFFVNTPD
INPVFLQTSGRPGFLIRAALAATLSGLWGVYNGFELCEGQALPGKEEYLDSEKYQLRAWD
HDRPGNIVAEIALLNRIRRANPALHSHLGLQFQPASGEGILYFSKSTPAPLPTHPAELAG
LTEGADAQPERFGDNTLLVAISLDPHHPQEADIELPLWQWGLPDHGALMAENLVDGTRFV
WRGKRQHLRLEPERPFVIWRVRPALPSEEQPS