Protein Info for RALBFv3_RS21450 in Ralstonia solanacearum IBSBF1503

Annotation: 2,3-diaminopropionate biosynthesis protein SbnB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR03944: 2,3-diaminopropionate biosynthesis protein SbnB
  • amino acids 9 to 338 (330 residues), 424 bits, E=1.8e-131
• PF02423: OCD_Mu_crystall
  • amino acids 29 to 328 (300 residues), 196.9 bits, E=2.1e-62

Best Hits

Swiss-Prot: 58% identical to SBNB_STAA8: N-((2S)-2-amino-2-carboxyethyl)-L-glutamate dehydrogenase (sbnB) from Staphylococcus aureus (strain NCTC 8325)

KEGG orthology group: K01750, ornithine cyclodeaminase [EC: 4.3.1.12] (inferred from 96% identity to rsl:RPSI07_mp0379)

MetaCyc: 58% identical to N-[(2S)-2-amino-2-carboxyethyl]-L-glutamate dehydrogenase monomer (Staphylococcus aureus aureus NCTC 8325)
RXN-18393 [EC: 1.5.1.51]

Predicted SEED Role

"Ornithine cyclodeaminase (EC 4.3.1.12) / Siderophore staphylobactin biosynthesis protein SbnB" in subsystem Arginine and Ornithine Degradation or Siderophore Staphylobactin (EC 4.3.1.12)

MetaCyc Pathways

• L-arginine degradation VII (arginase 3 pathway) (2/2 steps found)
• L-ornithine degradation I (L-proline biosynthesis) (1/1 steps found)
• L-proline biosynthesis II (from arginine) (1/2 steps found)
• staphyloferrin B biosynthesis (2/7 steps found)
• dapdiamides biosynthesis (2/13 steps found)
• zwittermicin A biosynthesis (2/13 steps found)
• sulfazecin biosynthesis (3/16 steps found)

KEGG Metabolic Maps

• Arginine and proline metabolism

Isozymes

Compare fitness of predicted isozymes for: 4.3.1.12

Use Curated BLAST to search for 1.5.1.51 or 4.3.1.12

Protein Sequence (339 amino acids)

>RALBFv3_RS21450 2,3-diaminopropionate biosynthesis protein SbnB (Ralstonia solanacearum IBSBF1503)
MTQSADTGLLYLGRQDLVALGGDRSQPYVDAITEGLALHAKQDFVQPLKPYLRWPGADHI
ADRIIAMPCYVGGKKPIAGLKWIGSRQHNPSRFQLERASAVIVLNDADTNYPVAIMEGGL
ISGMRTAAISAVATRYLAREGFTDVACIGCGPIARMQMQTLIEQFPGIRRVHLFDVSREA
MRGFSEAIAARFPQVACQVADSAEQAVRAADVIVTCTVTDAPYLEYAWLRRGAFVCNVSI
MDVHKEVYEKADKVVVDDWDQSNREKKIINQLVLEGRFSRERLHAELGEIVVGERPGREN
DDEIILLNPMGMAIDDMVCARHFYQLAEQAGVGTRLPLL