Protein Info for RALBFv3_RS20575 in Ralstonia solanacearum IBSBF1503

Annotation: FMN-dependent NADH-azoreductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 206 PF03358: FMN_red" amino acids 3 to 145 (143 residues), 31.6 bits, see alignment E=1.2e-11 PF02525: Flavodoxin_2" amino acids 3 to 200 (198 residues), 152.9 bits, see alignment E=9.7e-49

Best Hits

Swiss-Prot: 65% identical to AZOR1_BURL3: FMN-dependent NADH-azoreductase 1 (azoR1) from Burkholderia lata (strain ATCC 17760 / DSM 23089 / LMG 22485 / NCIMB 9086 / R18194 / 383)

KEGG orthology group: K01118, FMN-dependent NADH-azoreductase [EC: 1.7.-.-] (inferred from 65% identity to bur:Bcep18194_B0245)

Predicted SEED Role

"FMN-dependent NADH-azoreductase"

Isozymes

Compare fitness of predicted isozymes for: 1.7.-.-

Use Curated BLAST to search for 1.7.-.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (206 amino acids)

>RALBFv3_RS20575 FMN-dependent NADH-azoreductase (Ralstonia solanacearum IBSBF1503)
MSHILLITSSPRGADGLSTRFAGDLARKFAGQRPASTLTIRDLSAHPLAHIDAAYVDGRM
VAPEARTPAQAEAVSLAETLIAELRAADTIVIGSAMTNFGPSTQLRTWFDHVIWPGVTFN
YGSQSMEGLVTGKKVYLVTTSGGVFSEGPFVPLDYQAGYLKHLLGFIGMTDVELLRVEGT
AYGPEAAQAAIEKADAAVDALLAQAA