Protein Info for RALBFv3_RS20505 in Ralstonia solanacearum IBSBF1503

Annotation: membrane protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01903: CbiX
  • amino acids 6 to 110 (105 residues), 90.5 bits, E=7.9e-30
  • amino acids 136 to 239 (104 residues), 86.3 bits, E=1.6e-28
• PF02570: CbiC
  • amino acids 306 to 500 (195 residues), 222.8 bits, E=3.3e-70

Best Hits

KEGG orthology group: K06042, precorrin-8X methylmutase [EC: 5.4.1.2] (inferred from 95% identity to rsl:RPSI07_mp0507)

Predicted SEED Role

"Sirohydrochlorin cobaltochelatase (EC 4.99.1.3) / Cobalt-precorrin-8x methylmutase (EC 5.4.1.2)" in subsystem Cobalamin synthesis or Coenzyme B12 biosynthesis or Experimental tye (EC 4.99.1.3, EC 5.4.1.2)

MetaCyc Pathways

• adenosylcobalamin biosynthesis II (aerobic) (29/33 steps found)
• adenosylcobalamin biosynthesis I (anaerobic) (29/36 steps found)
• cob(II)yrinate a,c-diamide biosynthesis I (early cobalt insertion) (13/15 steps found)
• cob(II)yrinate a,c-diamide biosynthesis II (late cobalt incorporation) (11/13 steps found)
• siroheme biosynthesis (3/4 steps found)
• factor 430 biosynthesis (3/7 steps found)

KEGG Metabolic Maps

• Porphyrin and chlorophyll metabolism

Isozymes

Compare fitness of predicted isozymes for: 4.99.1.3

Use Curated BLAST to search for 4.99.1.3 or 5.4.1.2

Protein Sequence (523 amino acids)

>RALBFv3_RS20505 membrane protein (Ralstonia solanacearum IBSBF1503)
MVLAGHGSRDPDGIDEFMALVALLRARAGGGTVAHGFLEFATPTIDETVAAVVAGGAKTV
VMVPGVLLAATHAKNDMPSELLALKQAHPEVDFRFGAAMDLHPKLLALCRERIVEAEGRS
ARTVSRAESCLVVVGRGTSDPDANADIAKLTRMLEEGMGFGTSLVCYAGTARPLVADGLK
AAALLGYPRIVVLPYFLFDGVLVKRIYGAAADLAARHPELEVLTAPWLGVHPHVADVFLE
RAREGIEGRAAMNCALCKYRVQIVGFEEQVGTPQQGHHGLVRGLLGRNGTTPAPATDWPP
YVPHPIEAESMRIIDAGRDWSGVPADHQIVLKRLVHTSGDFDIVDDLYFSPGAVETGIRA
LLRCRRIVADVTMVQTGLKRAVLDQLGVETWCGVHDEETRLLAEAHGITRSAAGIRRAWQ
KFGNDVVVAIGDAPTAIVELVRLVREHGWRPQLVVGLPVGFVGTRESKEALRRLMQVPRI
TNRGTRGGSPWAASAVNALMIAAIDQVYRQTPSQQQQQPQQQA