Protein Info for RALBFv3_RS20320 in Ralstonia solanacearum IBSBF1503

Annotation: 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF08267: Meth_synt_1
  • amino acids 5 to 313 (309 residues), 390.7 bits, E=6.4e-121
• TIGR01371: 5-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase
  • amino acids 7 to 756 (750 residues), 1076.8 bits, E=0
• PF01717: Meth_synt_2
  • amino acids 153 to 330 (178 residues), 28 bits, E=1.4e-10
  • amino acids 430 to 752 (323 residues), 511.4 bits, E=1.1e-157

Best Hits

Swiss-Prot: 92% identical to METE_RALSO: 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase (metE) from Ralstonia solanacearum (strain GMI1000)

KEGG orthology group: K00549, 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase [EC: 2.1.1.14] (inferred from 93% identity to rsl:RPSI07_mp0577)

Predicted SEED Role

"5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase (EC 2.1.1.14)" in subsystem Methionine Biosynthesis (EC 2.1.1.14)

MetaCyc Pathways

• aspartate superpathway (23/25 steps found)
• superpathway of L-methionine biosynthesis (by sulfhydrylation) (12/12 steps found)
• superpathway of L-lysine, L-threonine and L-methionine biosynthesis I (16/18 steps found)
• superpathway of L-lysine, L-threonine and L-methionine biosynthesis II (13/15 steps found)
• L-methionine biosynthesis III (4/4 steps found)
• S-adenosyl-L-methionine salvage II (3/3 steps found)
• L-methionine biosynthesis II (5/6 steps found)
• superpathway of S-adenosyl-L-methionine biosynthesis (7/9 steps found)
• superpathway of L-methionine biosynthesis (transsulfuration) (7/9 steps found)
• superpathway of L-homoserine and L-methionine biosynthesis (6/8 steps found)
• S-adenosyl-L-methionine salvage I (3/4 steps found)
• L-methionine biosynthesis I (3/5 steps found)
• seleno-amino acid biosynthesis (plants) (3/5 steps found)
• superpathway of seleno-compound metabolism (6/19 steps found)

KEGG Metabolic Maps

• Biosynthesis of plant hormones
• Methionine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.1.1.14

Protein Sequence (776 amino acids)

>RALBFv3_RS20320 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase (Ralstonia solanacearum IBSBF1503)
MTTIHTLGYPRIGAQRELKFALESFWKGASTEADLRATGRTLRERHWNAQREAGLDLVTV
GDFAWYDQVLQTAALLGALPTRYGFDPARLTLAQSFVLARGNADHAAMEMTKWFDTNYHY
LVPELTPDLQDRQWGPGTGWLFDEVREAQAAGHRVKVVLLGPVTFLHLAKARGGLADKLS
LLPQLLQAYAAVLQRLATLGVEWVQIDEPALTLDLPKAWAEAFGPAYTTLAAANGPRLLL
ATYFEAASHHAALIKSLPVAGVHLDLVRAPQQLEAFAPWPADKILSAGVVDGRNIWRTDL
EHTLARITPLAQSLGERLWLAPSCSLLHVPVDLTAETRLDDELKGWLAFARQKLDELTLL
KRALAEGRAAAQDALADNAAAIASRAASRRVHNERVKKRVAAIRAQDAERAAPYPARAEA
QQARLNLPLLPTTTIGSFPQTAEIRQARAQYKRGELQALAYLERMRAEIADVVRRQEALG
LDMLVHGEAERNDMVEYFGELLWGYAFTANGWVQSYGSRCVKPPVIYGDVYRPEPMTVEW
SKYAQSLTSKPMKGMLTGPVTMLQWSFVRDDQPREQTALQIALALRDEVRDLEAAGIAAI
QIDEPAFREGLPLRTADVSGYLEWAARAFRVSASGVRNDTQIHTHMCYSEFNDILPAIAS
MDADVITIETSRSNMELLDAFGEFAYPNEIGPGVYDIHSPRVPRVEEMEALLDKAARVVP
VQRLWVNPDCGLKTRGWPEVEAALQGMVAATRRLRARHAGTVHAGTRAPQAESTIA